Role of heat shock protein 27 in regulation of glutathione system and apoptosis of Jurkat tumor cells and blood lymphocytes.

Abstract

Reaction of the glutathione system of Jurkat tumor cells and blood lymphocytes was evaluated under conditions of culturing with 5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl) isoxazole (KRIBB3), a selective inhibitor of heat shock protein Hsp27. The results indicated the regulatory role of Hsp27 in the maintenance of the functional activities of glutathione reductase, glutathione peroxidase, and realization of apoptotic death of Jurkat cells and blood lymphocytes. Inhibition of Hsp27 in Jurkat tumor cells led to imbalance of the glutathione system and increase of the share of annexin-positive cells.

• Authors: N V Ryazantseva, E A Stepovaya, O L Nosareva, E V Konovalova, D S Orlov, A I Naumova, S A Didenko, O N Vesnina, E V Shakhristova, A P Zima, V V Novitskii
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 158 Issue 3 Pg. 377-9 (Jan 2015) ISSN: 1573-8221 [Electronic] United States
• PMID: 25573372 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)isoxazole
• Anisoles
• HSP27 Heat-Shock Proteins
• Isoxazoles
• Glutathione Peroxidase
• Glutathione Reductase
• Glutathione

Topics

• Adult
• Animals
• Anisoles (pharmacology)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cells, Cultured
• Female
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• Glutathione Reductase (metabolism)
• HSP27 Heat-Shock Proteins (antagonists & inhibitors, genetics, metabolism)
• Humans
• Isoxazoles (pharmacology)
• Jurkat Cells (drug effects, metabolism)
• Lymphocytes (drug effects, metabolism)
• Male
• Oxidative Stress (drug effects)
• Young Adult