Abstract | OBJECTIVE: METHODS: From February 1, 2012, through October 31, 2012, preoperative urine samples were obtained from patients with a presumptive diagnosis of RCC based on an imaged renal mass, prostate cancer, or transitional cell bladder cancer. Imaged renal masses were diagnosed postnephrectomy—as malignant or benign—by histology. Urine AQP1 and PLIN2 concentrations were measured by using a sensitive and specific Western blot and normalized to urine creatinine concentration. RESULTS: Median concentrations of urine AQP1 and PLIN2 in patients with clear cell and papillary RCC (n=47) were 29 and 36 relative absorbance units/mg urine creatinine, respectively. In contrast, median concentrations in patients with bladder cancer (n=22) and prostate cancer (n=27), patients with chromophobe tumors (n=7), and patients with benign renal oncocytomas (n=9) and angiomyolipomas (n=7) were all less than 10 relative absorbance units/mg urine creatinine (Kruskal-Wallis test, P<.001 vs RCC for both biomarkers) and comparable with those in healthy controls. The area under the receiver operating characteristic curve ranged from 0.99 to 1.00 for both biomarkers. CONCLUSION: These results support the specificity and sensitivity of urine AQP1 and PLIN2 concentrations for RCC. These novel tumor-specific proteins have high clinical validity and high potential as specific screening biomarkers for clear cell and papillary RCC as well as in the differential diagnosis of imaged renal masses. TRIAL REGISTRATION: clinicaltrials.gov Identifier: NCT00851994.
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Authors | Jeremiah J Morrissey, Jonathan Mobley, R Sherburne Figenshau, Joel Vetter, Sam Bhayani, Evan D Kharasch |
Journal | Mayo Clinic proceedings
(Mayo Clin Proc)
Vol. 90
Issue 1
Pg. 35-42
(Jan 2015)
ISSN: 1942-5546 [Electronic] England |
PMID | 25572193
(Publication Type: Clinical Trial, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Mayo Foundation for Medical Education and Research. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- Membrane Proteins
- PLIN2 protein, human
- Perilipin-2
- Aquaporin 1
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Topics |
- Aged
- Aquaporin 1
(urine)
- Biomarkers, Tumor
(urine)
- Carcinoma, Renal Cell
(pathology, surgery, urine)
- Diagnosis, Differential
- Female
- Humans
- Kidney
(metabolism, pathology, surgery)
- Kidney Neoplasms
(pathology, surgery, urine)
- Male
- Membrane Proteins
(urine)
- Middle Aged
- Nephrectomy
(methods)
- Patient Outcome Assessment
- Perilipin-2
- Preoperative Care
(methods)
- ROC Curve
- Reproducibility of Results
- Sensitivity and Specificity
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