Abstract | OBJECTIVE: METHODS: Thirty rats were randomly divided into a control and a COPD group. The COPD model was established through smoking and lipopolysaccharide (LPS) tracheal instillation. Pulmonary function of the rats was measured 28 d after the establishment of the COPD model by a spirometer. Enzyme linked immunosorbent assay was performed to detect serum γ interferon (IFN-γ), interleukin (IL)-4, and IL-17. The expressions of T-bet, GATA-3, and RORgammat protein in lung tissues were determined by Western blot. RESULTS: Compared with the controls, the COPD rats had decreased pulmonary function and expression of serum IL-4, and increased INF-γ, IL- 17, Th1/Th2, T-bet, T bet /GATA-3, and RORgammat protein (P<0. 05). Forced expiratory volume in 0. 3 seconds (FEV 0.3) was negatively correlated with INF γ and T-bet/GATA-3. Forced vital capacity (FVC) was positively correlated with IL 4. FEV0.3/FVC was negatively correlated with Thl/Th2, T-bet and T-bet/GATA-3. Peak expiratory flow (PEF) was negatively correlated with IL-17, T bet, and RORgammat (P<0. 05). Thl/Th2 was positively correlated with T bet/GATA-3. IL-17 was positively correlated with RORgammat. T bet/GATA-3 was positively correlated with RORgammat (P<0. 05). CONCLUSION: Imbalanced regulation of T bet / GATA 3 and RORgammat on Th1/Th2 and Th17 cells is associated with the occurrence of COPD.
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Authors | Cheng-yangi Wang, Xiang-guo Liu, Chuan-bo Wang, Qiao Rue Ji, Li Fang, Ze Li Ze geng |
Journal | Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
(Sichuan Da Xue Xue Bao Yi Xue Ban)
Vol. 45
Issue 6
Pg. 941-5
(Nov 2014)
ISSN: 1672-173X [Print] China |
PMID | 25571720
(Publication Type: Journal Article)
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Chemical References |
- GATA3 Transcription Factor
- Interleukin-17
- Lipopolysaccharides
- Nuclear Receptor Subfamily 1, Group F, Member 3
- RNA, Messenger
- Rorc protein, rat
- T-Box Domain Proteins
- T-box transcription factor TBX21
- Interleukin-4
- Interferon-gamma
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Topics |
- Animals
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- GATA3 Transcription Factor
(metabolism)
- Interferon-gamma
(blood)
- Interleukin-17
(blood)
- Interleukin-4
(blood)
- Lipopolysaccharides
- Lung
(physiopathology)
- Nuclear Receptor Subfamily 1, Group F, Member 3
(metabolism)
- Pulmonary Disease, Chronic Obstructive
(immunology)
- RNA, Messenger
- Rats
- T-Box Domain Proteins
(metabolism)
- Th1-Th2 Balance
- Th17 Cells
(immunology)
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