GATA-binding protein 3 enhances the utility of gross cystic disease fluid protein-15 and mammaglobin A in triple-negative breast cancer by immunohistochemistry.

Abstract

AIMS:

We have demonstrated previously that gross cystic disease fluid protein-15 (GCDFP-15) and mammaglobin A (MAM) are of limited utility in triple-negative breast cancer (TNBC). GATA-binding protein 3 (GATA-3) is an emerging breast-associated immunohistochemical (IHC) marker with limited data in TNBC. Here, we examined GATA-3 expression in TNBC in comparison with GCDFP-15 and MAM.

METHODS AND RESULTS:

We studied GATA-3, GCDFP-15 and MAM IHC expression in 62 primary and 68 metastatic TNBCs. In primary TNBCs, GATA-3 staining was observed in 25 cases (40%), including 16 cases that were negative for GCDFP-15 and MAM. In metastatic TNBCs, GATA-3 staining was observed in 30 cases (44%), including 16 cases that were negative for GCDFP-15 and MAM. The expression frequency of any of the markers was 56% in primary and 62% in metastatic TNBCs. However, when focal staining was excluded, the expression frequency of any marker dropped to 31% and 44%, respectively.

CONCLUSION:

GATA-3 is expressed at a higher frequency by IHC in TNBC compared to GCDFP-15 and MAM, although the tissue specificity of the latter markers may be superior. When evaluating a triple-negative tumour, including GATA-3 in a panel of markers may increase the diagnostic accuracy for tissue origin in the appropriate clinical setting.

• Authors: Lei Huo, Yun Gong, Ming Guo, Michael Z Gilcrease, Yun Wu, Hong Zhang, Jinxia Zhang, Erika Resetkova, Kelly K Hunt, Michael T Deavers
• Journal: Histopathology (Histopathology) Vol. 67 Issue 2 Pg. 245-54 (Aug 2015) ISSN: 1365-2559 [Electronic] England
• PMID: 25564996 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 John Wiley & Sons Ltd.

Chemical References

• Biomarkers, Tumor
• Carrier Proteins
• GATA3 Transcription Factor
• GATA3 protein, human
• Glycoproteins
• Mammaglobin A
• Membrane Transport Proteins
• Neoplasm Proteins
• PIP protein, human

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Carrier Proteins (metabolism)
• Female
• GATA3 Transcription Factor (metabolism)
• Glycoproteins (metabolism)
• Humans
• Immunoenzyme Techniques (methods)
• Mammaglobin A (metabolism)
• Membrane Transport Proteins
• Middle Aged
• Neoplasm Proteins (metabolism)
• Staining and Labeling
• Triple Negative Breast Neoplasms (metabolism, pathology)