Involvement of decreased neuroglobin protein level in cognitive dysfunction induced by 1-bromopropane in rats.

Abstract

1-Bromopropane (1-BP) is used as a substitute for ozone-depleting solvents (ODS) in industrial applications. 1-BP could display central nervous system (CNS) neurotoxicity manifested by cognitive dysfunction. Neuroglobin (Ngb) is an endogenous neuroprotectant and is predominantly expressed in the nervous system. The present study aimed to investigate Ngb involvement in CNS neurotoxicity induced by 1-BP in rats. Male Wistar rats were randomly divided into 5 groups (n=14) and treated with 0, 100, 200, 400 and 800 mg/kg bw 1-BP, respectively, by gavage for consecutive 12 days. Rats displayed cognitive dysfunction dose-dependently through Morris water maze (MWM) test. Significant neuron loss in layer 5 of the prelimbic cortex (PL) was observed. Moreover, 1-BP decreased Ngb protein level in cerebral cortex and Ngb decrease was significantly positively correlated with cognitive dysfunction. Glutathione (GSH) content, GSH/oxidized glutathione (GSSG) ratio and glutamate cysteine ligase (GCL) activity decreased in cerebral cortex, coupled with the increase in GSSG content. GSH and GSH/GSSG ratio decrease were significantly positively correlated with cortical Ngb decrease. Additionally, levels of N-epsilon-hexanoyl-lysine (HEL) and 4-hydroxy-2-nonenal (4-HNE) modified proteins in cerebral cortex of 1-BP-treated rats increased significantly. In conclusion, it was suggested that 1-BP resulted in decreased endogenous neuroprotectant Ngb in cerebral cortex, which might play an important role in CNS neurotoxicity induced by 1-BP and that 1-BP-induced oxidative stress in cerebral cortex might partly be responsible for Ngb decrease.

• Authors: Ying Guo, Hua Yuan, Lulu Jiang, Junlin Yang, Tao Zeng, Keqin Xie, Cuili Zhang, Xiulan Zhao
• Journal: Brain research (Brain Res) Vol. 1600 Pg. 1-16 (Mar 10 2015) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 25557405 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Hydrocarbons, Brominated
• Nerve Tissue Proteins
• Neuroglobin
• Globins
• 1-bromopropane

Topics

• Animals
• Cognition Disorders (chemically induced)
• Globins (metabolism)
• Hydrocarbons, Brominated (administration & dosage, toxicity)
• Male
• Maze Learning (drug effects)
• Nerve Tissue Proteins (metabolism)
• Neuroglobin
• Neurons (drug effects)
• Oxidative Stress (drug effects)
• Prefrontal Cortex (drug effects, metabolism)
• Rats
• Rats, Wistar