Expression of syntaxin 8 in visceral adipose tissue is increased in obese patients with type 2 diabetes and related to markers of insulin resistance and inflammation.

Abstract

BACKGROUND AND AIMS:

Obesity is associated with increased adipose tissue inflammation as well as with the development of type 2 diabetes (T2D). Syntaxin 8 (STX8) is a protein required for the transport of endosomes. In this study we analyzed the relationship of STX8 with the presence of T2D in the context of obesity.

METHODS:

With this purpose, 21 subjects (seven lean [LN], eight obese normoglycemic [OB-NG] and six obese with type 2 diabetes [OB-T2D]) were included in the study. Gene and protein expression levels of STX8 and GLUT4 were analyzed in visceral adipose tissue (VAT).

RESULTS:

mRNA (p = 0.008) and protein (p <0.001) expression levels of STX8 were significantly increased in VAT of OB-T2D patients. Moreover, gene expression levels of SLC2A4 (GLUT4) were downregulated (p = 0.002) in VAT of obese patients. We found that STX8 was positively correlated (p <0.05) with fasting glucose concentrations, plasma glucose 2 h after an OGTT and C-reactive protein. Interestingly, the expression of STX8 was negatively correlated (p <0.05) with the expression of SLC2A4 in VAT.

CONCLUSIONS:

Increased STX8 expression in VAT appears to be associated with the presence of T2D in obese patients through a mechanism that may involve GLUT4.

• Authors: Andoni Lancha, Santiago López-Garrido, Amaia Rodríguez, Victoria Catalán, Beatriz Ramírez, Víctor Valentí, Rafael Moncada, Camilo Silva, María J Gil, Javier Salvador, Gema Frühbeck, Javier Gómez-Ambrosi
• Journal: Archives of medical research (Arch Med Res) Vol. 46 Issue 1 Pg. 47-53 (Jan 2015) ISSN: 1873-5487 [Electronic] United States
• PMID: 25523146 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 IMSS. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• Blood Glucose
• Glucose Transporter Type 4
• Qa-SNARE Proteins
• RNA, Messenger
• SLC2A4 protein, human
• C-Reactive Protein

Topics

• Adult
• Biomarkers (metabolism)
• Blood Glucose (analysis)
• C-Reactive Protein (metabolism)
• Diabetes Mellitus, Type 2 (pathology)
• Female
• Gene Expression
• Glucose Transporter Type 4 (biosynthesis)
• Humans
• Inflammation (immunology)
• Insulin Resistance (genetics)
• Intra-Abdominal Fat (metabolism)
• Male
• Middle Aged
• Obesity (metabolism)
• Qa-SNARE Proteins (biosynthesis)
• RNA, Messenger (genetics)