Activated fibronectin-secretory phenotype of mesenchymal stromal cells in pre-fibrotic myeloproliferative neoplasms.

Abstract

We characterized bone marrow stromal cells (BMSC) from patients with pre-fibrotic myeloproliferative neoplasms (MPN). MPN-BMSC showed decreased capacity to stimulate the proliferation of colony-forming units of normal hematopoietic stem and progenitor cells and displayed increased matrix remodelling (in particular fibronectin deposition) compared to control BMSC. This finding was confirmed in pre-fibrotic MPN bone marrow biopsies in a tissue microarray (n = 34), which stained positive for fibronectin in the absence of reticulin as a standard myelofibrosis marker. Fibronectin expression correlated significantly with reduced haemoglobin levels in MPN-patients (p = 0.007; R2 = 0.42). Our data show significant cell-intrinsic alterations in MPN-MSC and suggest that Fibronectin expression might be applicable as a biomarker for the identification of early myelofibrotic transformation in reticulin-negative MPN.

• Authors: Rebekka K Schneider, Susanne Ziegler, Isabelle Leisten, Monica S V Ferreira, Anne Schumacher, Björn Rath, Dirk Fahrenkamp, Gerhard Müller-Newen, Martina Crysandt, Stefan Wilop, Edgar Jost, Steffen Koschmieder, Ruth Knüchel, Tim H Brümmendorf, Patrick Ziegler
• Journal: Journal of hematology & oncology (J Hematol Oncol) Vol. 7 Pg. 92 (Dec 14 2014) ISSN: 1756-8722 [Electronic] England
• PMID: 25498831 (Publication Type: Letter, Research Support, Non-U.S. Gov't)

Chemical References

• Fibronectins

Topics

• Fibronectins (metabolism)
• Humans
• Mesenchymal Stem Cells (metabolism, pathology)
• Myeloproliferative Disorders (genetics, pathology)
• Phenotype
• Tissue Array Analysis