Co-carcinogenic effects of NaHCO3 on o-phenylphenol-induced rat bladder carcinogenesis.

Abstract

The role of urinary pH and Na+ concentration on the bladder carcinogenesis of o-phenylphenol (OPP) was examined in male F344 rats. The rats were given powdered diet containing 2% sodium o-phenylphenate (OPP-Na, group 1), 1.25% OPP plus 0.64% NaHCO3 (group 2), 1.25% OPP plus 0.32% NaHCO3 (group 3), 1.25% OPP plus 0.16% NaHCO3 (group 4), 1.25% OPP (group 5), 0.64% NaHCO3 (group 6) or no test chemical (group 7) for 104 weeks respectively. Incidences of bladder carcinoma induced were significantly higher in groups 1 (12 of 29 rats, 41.4%) and 2 (9 of 29 rats, 31.0%) than in group 7 (0 of 27 rats, 0%). Groups 3 and 4 induced bladder carcinomas in 4 of 29 rats (13.8%) and 4 of 26 rats (15.4%) respectively, whereas no tumors occurred in group 5 (0 of 27, 0%). The incidence in group 6 was 3.6% (1 of 28 rats). Groups 1 and 2 induced significant increases in urinary pH and Na+ concentrations, whereas group 5 did not. Groups 3 and 4 showed the same tendency as groups 1 and 2. Examination with a scanning electron microscope showed the appearance of pleomorphic microvilli, short, uniform microvilli, and ropy or leafy microridges on the luminal surface of the bladder in groups 1-5 of rats treated with OPP or OPP-Na for 8 weeks. The appearance and severity were the same in groups 1 and 2, followed by the groups with decreasing doses of NaHCO3. The results indicated that OPP-Na is carcinogenic for the rat bladder, but OPP is not. However, increased urinary pH and Na+ concentration play important roles in OPP-Na rat bladder carcinogenesis.

• Authors: S Fukushima, T Inoue, S Uwagawa, M A Shibata, N Ito
• Journal: Carcinogenesis (Carcinogenesis) Vol. 10 Issue 9 Pg. 1635-40 (Sep 1989) ISSN: 0143-3334 [Print] England
• PMID: 2548750 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bicarbonates
• Biphenyl Compounds
• Carcinogens
• Fungicides, Industrial
• Sodium Bicarbonate
• Sodium
• 2-phenylphenol

Topics

• Animals
• Bicarbonates (toxicity)
• Biphenyl Compounds (toxicity)
• Carcinogens
• Carcinoma (chemically induced, pathology)
• Fungicides, Industrial (toxicity)
• Hydrogen-Ion Concentration
• Hyperplasia
• Male
• Papilloma (chemically induced, pathology)
• Rats
• Rats, Inbred F344
• Sodium (toxicity)
• Sodium Bicarbonate
• Urinary Bladder (drug effects, pathology)
• Urinary Bladder Neoplasms (chemically induced, pathology)
• Urine