Efficacy of dual-functional liposomes containing paclitaxel for treatment of lung cancer.

Abstract

This study was mainly focused on the development of a dual-ligand liposomal delivery system for targeting the delivery of paclitaxel (PTX) to lung cancer. The specific ligand peptide HAIYPRH (T7) and the cationic cell-penetrating peptide TAT were connected with phospholipid via a polyethylene glycol (PEG) spacer to prepare the dual-ligand liposomes (T7/TAT-LP-PTX). Physicochemical characterizations of T7/TAT-LP-PTX, such as particle size, ζ potential, morphology, encapsulation efficiency, and in vitro PTX release, were also evaluated. In the cellular uptake study, the T7/TAT-LP endocytosed by the A549 cells was 2.26-, 3.48- and 8.56-fold higher than TAT-LP, T7-LP and LP, respectively. The IC50 values of TAT-LP-PTX, T7-LP-PTX and LP-PTX were much higher than those of T7/TAT-LP-PTX, respectively. The homing specificity of T7/TAT-LP was evaluated on the tumor spheroids, which revealed that T7/TAT-LP was more efficaciously internalized in tumor cells than TAT-LP, T7-LP and LP, respectively. Compared to LP, TAT-LP and T7-LP, T7/TAT-LP showed the strongest cell uptake property, and the highest accumulation ability in tumor spheroids in vitro. In the in vivo study, the T7/TAT-LP-PTX exhibited the best inhibitory effect of tumor growth for A549-bearing mice. Collectively, these results suggested that T7/TAT-LP-PTX is a promising drug delivery system for the treatment of lung cancer.

• Authors: Rong-Hua Wang, Hong-Mei Cao, Zhi-Ju Tian, Bo Jin, Qing Wang, Hong Ma, Jing Wu
• Journal: Oncology reports (Oncol Rep) Vol. 33 Issue 2 Pg. 783-91 (Feb 2015) ISSN: 1791-2431 [Electronic] Greece
• PMID: 25482610 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Cell-Penetrating Peptides
• Liposomes
• Polyethylene Glycols
• Paclitaxel

Topics

• Animals
• Cell Line, Tumor
• Cell-Penetrating Peptides (chemistry)
• Drug Delivery Systems
• Humans
• Liposomes (administration & dosage, chemistry)
• Lung Neoplasms (drug therapy)
• Male
• Mice
• Mice, Inbred BALB C
• Paclitaxel (administration & dosage, pharmacokinetics)
• Particle Size
• Polyethylene Glycols (chemistry)
• Signal Transduction (drug effects)
• Xenograft Model Antitumor Assays