Gallic acid inhibits vascular calcification through the blockade of BMP2-Smad1/5/8 signaling pathway.

Abstract

Vascular calcification is associated with increased risk of morbidity and mortality in patients with cardiovascular diseases, chronic kidney diseases, and diabetes. Gallic acid, a natural compound found in gallnut and green tea, is known to be antifungal, antioxidant, and anticancer. Here we investigated the effect of gallic acid on vascular smooth muscle cell (VSMC) calcification and the underlying mechanism. Gallic acid inhibited inorganic phosphate-induced osteoblast differentiation markers as well as calcification phenotypes (as determined by calcium deposition, Alizarin Red, and Von Kossa staining). Knockdown of BMP2 or Noggin blocked phosphate-induced calcification. Gallic acid suppressed phosphorylation of Smad1/5/8 protein induced by inorganic phosphate. Taken together, we suggest that gallic acid acts as a novel therapeutic agent of vascular calcification by mediating BMP2-Smad1/5/8 signaling pathway.

• Authors: Hae Jin Kee, Soo-Na Cho, Gwi Ran Kim, Sin Young Choi, Yuhee Ryu, In Kyeom Kim, Young Joon Hong, Hyung Wook Park, Youngkeun Ahn, Jeong Gwan Cho, Jong Chun Park, Myung Ho Jeong
• Journal: Vascular pharmacology (Vascul Pharmacol) Vol. 63 Issue 2 Pg. 71-8 (Nov 2014) ISSN: 1879-3649 [Electronic] United States
• PMID: 25446167 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Bmp2 protein, rat
• Bone Morphogenetic Protein 2
• Smad Proteins
• Gallic Acid

Topics

• Animals
• Bone Morphogenetic Protein 2 (antagonists & inhibitors)
• Cell Differentiation (drug effects)
• Cell Line
• Gallic Acid (pharmacology)
• Muscle, Smooth, Vascular (drug effects)
• Myocytes, Smooth Muscle (drug effects)
• Osteoblasts (drug effects)
• Phosphorylation (drug effects)
• Rats
• Signal Transduction (drug effects)
• Smad Proteins (antagonists & inhibitors)
• Vascular Calcification (drug therapy)