B7-H4 expression is correlated with tumor progression and clinical outcome in urothelial cell carcinoma.

Abstract

OBJECTIVE:

To investigate the mRNA and protein levels of B7-H4, a B7 family molecule, in human urothelial cell carcinoma (UCC), to analyze the relationship between B7-H4 protein expression level and pathological stage of UCC, and to examine the potential of B7-H4 as a prognostic factor in UCC.

METHODS:

mRNA and protein levels of B7-H4 were measured in pairs of tumor tissues and matched adjacent nontumor tissue obtained from patients with UCC by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical staining, respectively. Association of the protein level of B7-H4 with pathological tumor stage and the overall survival of UCC patients were also analyzed.

RESULTS:

B7-H4 mRNA and protein level were significantly higher in UCC tumor tissues compared with adjacent nontumor tissues as assessed by qRT-PCR and immunohistochemical staining, respectively. Higher B7-H4 protein levels were observed in patients with more advanced pathological stage of UCC and were also associated with decreased overall survival of patients with UCC.

CONCLUSIONS:

The findings from this study indicate that B7-H4 has the potential to be an independent prognostic indicator for UCC.

• Authors: Min Fan, Qianfeng Zhuang, Yiming Chen, Tao Ding, Hongwei Yao, Lujun Chen, Xiaozhou He, Xianlin Xu
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 7 Issue 10 Pg. 6768-75 ( 2014) ISSN: 1936-2625 [Electronic] United States
• PMID: 25400757 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• RNA, Messenger
• V-Set Domain-Containing T-Cell Activation Inhibitor 1
• VTCN1 protein, human

Topics

• Biomarkers, Tumor (analysis, genetics)
• Carcinoma (chemistry, genetics, mortality, pathology, surgery)
• Case-Control Studies
• Disease Progression
• Disease-Free Survival
• Female
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Male
• Neoplasm Staging
• Predictive Value of Tests
• Proportional Hazards Models
• RNA, Messenger (analysis)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Time Factors
• Treatment Outcome
• Urinary Bladder Neoplasms (chemistry, genetics, mortality, pathology, surgery)
• Urothelium (chemistry, pathology, surgery)
• V-Set Domain-Containing T-Cell Activation Inhibitor 1 (analysis, genetics)