[Adenovirus-mediated interleukin-24 enhances the inhibitory effect of paclitaxel on the growth of lung cancer A549 cells].

Abstract

OBJECTIVE:

To investigate the effect of adenovirus-mediated interleukin-24 (Ad-IL-24) combined with paclitaxel on the growth of human lung cancer A549 cells in vitro.

METHODS:

A549 cells were treated with Ad-IL-24 alone, paclitaxel alone and Ad-IL-24 combined with paclitaxel, respectively. Their proliferation was detected by CCK-8 assay, and the changes of apoptosis and cell cycle were tested by flow cytometry.

RESULTS:

The treatment of Ad-IL-24 significantly inhibited the A549 cell growth and induced cell apoptosis. Compared with paclitaxel alone and Ad-IL-24 alone, the Ad-IL-24 plus paclitaxel treatment more evidently inhibited lung cancer cell growth and increased cell apoptosis rate, and induced G2/M phase arrest.

CONCLUSION:

Ad-IL-24 infection can enhance the inhibitory effect of paclitaxel on lung cancer cell growth.

• Authors: Mingju Xu, Minmin Li, Jicheng Yang, Jinjin Guo, Wanbang Sun
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 30 Issue 11 Pg. 1150-3 (Nov 2014) ISSN: 1007-8738 [Print] China
• PMID: 25374078 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Interleukins
• interleukin-24
• Paclitaxel

Topics

• Adenoviridae (genetics)
• Antineoplastic Agents, Phytogenic
• Apoptosis
• Cell Cycle Checkpoints (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Combined Modality Therapy
• Genetic Therapy
• Humans
• Interleukins (genetics)
• Lung Neoplasms (pathology, therapy)
• Paclitaxel (pharmacology)