Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS:
Ovarian cancer cells in primary tumors and ascites expressed markers of cancer stem cells and markers of both mesenchymal and epithelial cells. Expression of HERV transcripts and HERV-K ENV protein and reverse transcriptase activities were higher in ovarian cancer compared with adjacent normal and benign tissues. The ovarian cancer patient plasma also had high reverse transcriptase activities and the ovarian cancer patient sera contained HERV-K immunoreactive antibodies. HERV-K-specific T cells generated from autologous dendritic cells pulsed with HERV-K ENV antigens exhibited phenotypes and functions consistent with a cellular immune response including T-cell proliferation, IFNγ production, and HERV-K-specific cytotoxic T lymphocyte (CTL) activity. Significantly higher CTL lysis of autologous tumor cells than of uninvolved normal cells was demonstrated in patients with ovarian cancer than patients with benign diseases and further enhanced lysis was observed if T regulatory cells were depleted. CONCLUSION:
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Authors | Kiera Rycaj, Joshua B Plummer, Bingnan Yin, Ming Li, Jeremy Garza, Laszlo Radvanyi, Lois M Ramondetta, Kevin Lin, Gary L Johanning, Dean G Tang, Feng Wang-Johanning |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 21
Issue 2
Pg. 471-83
(Jan 15 2015)
ISSN: 1557-3265 [Electronic] United States |
PMID | 25370465
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | ©2014 American Association for Cancer Research. |
Chemical References |
- Antigens, Neoplasm
- Gene Products, env
- RNA-Directed DNA Polymerase
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Topics |
- Antigens, Neoplasm
(blood, genetics, metabolism)
- Cell Line, Tumor
- Cell Proliferation
- Cytotoxicity, Immunologic
- Endogenous Retroviruses
(genetics, metabolism)
- Female
- Gene Products, env
(blood, genetics)
- Humans
- Lymphocyte Activation
- Ovarian Neoplasms
(blood, virology)
- RNA-Directed DNA Polymerase
(blood, genetics)
- T-Lymphocytes, Cytotoxic
(immunology, virology)
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