Self-adjuvanting therapeutic peptide-based vaccine induce CD8+ cytotoxic T lymphocyte responses in a murine human papillomavirus tumor model.

Abstract

Vaccine candidates for the treatment of human papillomavirus (HPV)-associated cancers are aimed to activate T-cells and induce development of cytotoxic anti-tumor specific responses. Peptide epitopes derived from HPV-16 E7 oncogenic protein have been identified as promising antigens for vaccine development. However, peptide-based antigens alone elicit poor cytotoxic T lymphocyte (CTL) responses and need to be formulated with an adjuvant (immunostimulant) to achieve the desired immune responses. We have reported the ability of polyacrylate 4-arm star-polymer (S4) conjugated with HPV-16 E744-57 (8Qmin) epitope to reduce and eradicate TC-1 tumor in the mouse model. Herein, we have studied the mechanism of induction of immune responses by this polymer-peptide conjugate and found prompt uptake of conjugate by antigen presenting cells, stimulating stronger CD8(+) rather than CD4(+) or NK cell responses.

• Authors: Tzu-Yu Liu, Ashwini Kumar Giddam, Waleed M Hussein, Zhongfan Jia, Nigel A J McMillan, Michael J Monteiro, Istvan Toth, Mariusz Skwarczynski
• Journal: Current drug delivery (Curr Drug Deliv) Vol. 12 Issue 1 Pg. 3-8 ( 2015) ISSN: 1875-5704 [Electronic] United Arab Emirates
• PMID: 25269453 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acrylic Resins
• Adjuvants, Immunologic
• Cancer Vaccines
• Drug Carriers
• Epitopes
• Papillomavirus E7 Proteins
• Papillomavirus Vaccines
• oncogene protein E7, Human papillomavirus type 16
• carbopol 940

Topics

• Acrylic Resins (chemistry)
• Adjuvants, Immunologic (administration & dosage, chemistry)
• Animals
• Cancer Vaccines (administration & dosage, chemistry, immunology)
• Cell Line, Tumor
• Chemistry, Pharmaceutical
• Drug Carriers
• Epitopes
• Female
• Immunization
• Lung Neoplasms (drug therapy, immunology, virology)
• Mice, Inbred C57BL
• Molecular Structure
• Papillomavirus E7 Proteins (administration & dosage, chemistry, immunology)
• Papillomavirus Infections (drug therapy, immunology, virology)
• Papillomavirus Vaccines (administration & dosage, chemistry, immunology)
• T-Lymphocytes, Cytotoxic (drug effects, immunology, virology)
• Technology, Pharmaceutical (methods)