Abstract |
Newcastle disease viruses (NDV) isolated from pigeons (pigeon paramyxovirus type 1; PPMV-1) are mostly of mesogenic pathotype and characterized by a polybasic amino acid sequence motif at the fusion protein (F) cleavage site. This feature also applies to isolate R75/98 from Germany. Its genome consists of 15,192 nucleotides and it specifies an intracerebral pathogenicity index (ICPI) of 1.1, as is typical for mesogenic NDV. Recombinant R75/98 (rR75/98) derived by reverse genetics also possesses a polybasic F protein cleavage site but exhibits ICPI of 0.28, indicating a lentogenic virus. While ten virus passages of rR75/98 on embryonated chicken eggs did not result in any alteration of virus characteristics, virus which had been re-isolated from the brain of an intracerebrally inoculated chicken showed an increase in virulence, characterized by an ICPI of 0.93. Comparison of whole genome sequences of rR75/98 and re-isolated rR75/98 (RrR75/98) demonstrated only two amino acid differences, one in the F protein (N472 K) and one in the polymerase protein (K2168R). This result indicates that only very few amino acid alterations are sufficient to modulate virus virulence in the presence of a polybasic amino acid sequence at the proteolytic F protein cleavage site.
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Authors | Sandra Heiden, Christian Grund, Dirk Höper, Thomas C Mettenleiter, Angela Römer-Oberdörfer |
Journal | Virus genes
(Virus Genes)
Vol. 49
Issue 3
Pg. 502-6
(Dec 2014)
ISSN: 1572-994X [Electronic] United States |
PMID | 25228150
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Viral
- Viral Fusion Proteins
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Topics |
- Animals
- Chick Embryo
- Columbidae
- Genome, Viral
- Germany
- Molecular Sequence Data
- Mutation, Missense
- Newcastle Disease
(virology)
- Newcastle disease virus
(classification, genetics, isolation & purification, pathogenicity)
- RNA, Viral
(genetics)
- Sequence Analysis, DNA
- Serial Passage
- Viral Fusion Proteins
(genetics)
- Virulence
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