Yizhijiannao granules have been shown to improve cognitive function in
Alzheimer's disease patients. The present study sought to explore the mechanisms involved in the cognitive enhancing effects of Yizhijiannao granule. Senescence-accelerated mouse prone 8 mice with learning and
memory disorders were intragastrically treated with Yizhijiannao granule for 8 weeks. Mice intragastrically treated with double distilled water for 8 weeks were considered as the control group. 2D gel electrophoresis was used to isolate total
protein from the temporal lobe of senescence-accelerated mouse prone 8 mice, and differential
protein spots were obtained by mass spectrometry. Thirty-seven differential
protein spots were found in the temporal lobe area of both groups. Ten
protein spots were identified: high mobility group box 1,
dimethylarginine dimethylaminohydrolase-1,
neuroglobin,
hemoglobin beta adult major chain, peroxiredoxin-6, cofilin-1,
flotillin 1,
peptidylprolyl isomerase A, voltage-dependent
anion channel-2 and
chaperonin containing TCP1, and subunit 2. Among other functions, these
proteins are separately involved in the regulation of
amyloid beta production, oxidative stress,
neuroinflammation, regulation of tau phosphorylation, and regulation of neuronal apoptosis. Our results revealed that Yizhijiannao granule can regulate the expression of various
proteins in the temporal lobe of senescence-accelerated mouse prone 8 mice, and may be therapeutically beneficial for the treatment of
Alzheimer's disease.