Eye-mediated immune tolerance to Type II collagen in arthritis-prone strains of mice.

Abstract

Type II collagen (CII) is a cartilage structural protein that plays important roles in joint function, arthritis and ageing. In studying the ability of CII to induce eye-mediated specific immune tolerance, we have recently proven that CII is capable of inducing anterior chamber-associated immune deviation (ACAID) in Balb/c mice. Here, we study the ability of CII to induce eye-mediated immune tolerance in strains of mice that are prone to the induction of rheumatoid arthritis. Thus, we hypothesized that CII induces ACAID in DBA/1 mice and in C57BL/6 mice through the AC route (direct injection) or the intravenous route (adoptive transfer of in vitro-generated CII-specific ACAID macrophages or of CII-specific in vitro-generated T regulatory cells). Specific immune tolerance induction was assessed using both delayed-type hypersensitivity (DTH) and local adoptive transfer (LAT) assays. Results indicated the ability of CII to generate CII-specific ACAID-mediated immune tolerance in vivo and in vitro in both DBA/1 mice and C57BL/6 mice. These findings could be beneficial in studies of immune tolerance induction using CII.

• Authors: Shukkur M Farooq, Ashok Kumar, Hossam M Ashour
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 18 Issue 12 Pg. 2512-8 (Dec 2014) ISSN: 1582-4934 [Electronic] England
• PMID: 25211510 (Publication Type: Journal Article)
• Copyright: © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• Collagen Type II

Topics

• Adoptive Transfer (methods)
• Animals
• Anterior Chamber (drug effects, immunology)
• Arthritis (immunology, metabolism)
• Cells, Cultured
• Collagen Type II (administration & dosage, immunology, metabolism)
• Eye (drug effects, immunology)
• Hypersensitivity, Delayed (immunology, metabolism)
• Immune Tolerance (immunology)
• Immunization (methods)
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Spleen (cytology, immunology)
• T-Lymphocytes, Regulatory (immunology, transplantation)