Abstract | OBJECTIVE: METHODS: Female apolipoprotein E-deficient mice (a well-established model of atherogenesis) were ovariectomized or falsely operated and fed either standard diet or high-fat diet (HFD). Six animals were included in each of the four groups. To clarify mechanisms, we treated preosteoblastic MC3T3-E1 cells with mouse serum. RESULTS: Physiological levels of estrogens in falsely operated mice limited atherosclerotic burden in the thoracic aorta, but not in the aortic arch. Bone resorption, as assessed by C-telopeptides, was increased by ovariectomy in animals fed standard diet, but not in animals fed HFD. Bone microstructural properties at the distal femur showed deteriorated trabecular architecture in bone volumetric fraction and trabecular number after ovariectomy, but trabecular pattern factor, trabecular thickness, trabecular spacing, or the structural model index remained unchanged. Changes in cortical parameters were not significant. Volumetric bone mineral density was reduced in trabecular bone, but not in cortical bone, in ovariectomized mice fed standard diet. Preosteoblastic MC3T3-E1 cells exhibited increased cellular proliferation and viability and alkaline phosphatase activity after treatment with sera from animals fed HFD. CONCLUSIONS:
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Authors | María Leonor Fernández-Murga, Ángela Vinué, José Ramón Caeiro, David Guede, Juan J Tarín, Vicente Andrés, Antonio Cano |
Journal | Menopause (New York, N.Y.)
(Menopause)
Vol. 22
Issue 4
Pg. 428-36
(Apr 2015)
ISSN: 1530-0374 [Electronic] United States |
PMID | 25203894
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoproteins E
- Estrogens
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Topics |
- Animals
- Apolipoproteins E
(deficiency)
- Atherosclerosis
(metabolism, prevention & control)
- Bone Density
- Bone Resorption
- Diet, High-Fat
- Disease Models, Animal
- Estrogens
(physiology)
- Female
- Humans
- Mice
- Osteoporosis
(prevention & control)
- Ovariectomy
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