Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra.

Abstract

Escherichia coli is the most common bacterial agent isolated from canine pyometra. The frequencies of 24 virulence genes and pulsed field gel electrophoresis (PFGE) profiles were determined for 23 E. coli isolates from cases of canine pyometra in Brazil. The frequencies of virulence genes were 91.3% fimH, 91.3% irp-2, 82.6% fyuA, 56.5% iroN, 47.8% traT, 39.1% usp, 34.8% sfaD/E, 34.8% tsh, 30.4% papC, 30.4% hlyA, 26.1% papGIII, 26.1% cnf-1, 21.7% papE/F, 21.7% iss, 17.4% iutA, 17.4% ompT, 17.4% cvaC, 17.4% hlyF, 17.4% iucD, 13.0% iucC, 13.0% astA, 4.3% papGII, 0% afaB/C and 0% papGI. The high frequency of yersiniabactin (fyuA and irp2) and salmochelin (iroN) genes suggests that iron uptake systems might be important in the pathogenesis of canine pyometra. PFGE profiles of 19 isolates were heterogeneous, confirming that E. coli isolates from canine pyometra are unlikely to be epidemic clones.

• Authors: Renato P Maluta, Clarissa A Borges, Lívia G Beraldo, Marita V Cardozo, Fabiana A Voorwald, André M Santana, Everlon C Rigobelo, Gilson H Toniollo, Fernando A Avila
• Journal: Veterinary journal (London, England : 1997) (Vet J) Vol. 202 Issue 2 Pg. 393-5 (Nov 2014) ISSN: 1532-2971 [Electronic] England
• PMID: 25201253 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Bacterial Proteins

Topics

• Animals
• Bacterial Proteins (genetics)
• Clone Cells (microbiology)
• DNA Fingerprinting (veterinary)
• Dog Diseases (microbiology)
• Dogs
• Electrophoresis, Gel, Pulsed-Field
• Escherichia coli (genetics, pathogenicity)
• Female
• Molecular Sequence Data
• Phylogeny
• Polymerase Chain Reaction (veterinary)
• Pyometra (microbiology, veterinary)
• Sequence Analysis, DNA (veterinary)
• Virulence (genetics)