Abstract | INTRODUCTION: Preterm newborns often require mechanical respiratory support that can result in ventilation-induced lung injury (VILI), despite exogenous surfactant treatment. Human amnion epithelial cells (hAECs) reduce lung inflammation and resultant abnormal lung development in preterm animals; co-administration with surfactant is a potential therapeutic strategy. We aimed to determine whether hAECs remain viable and maintain function after combination with surfactant. METHODS: hAECs were incubated in surfactant ( Curosurf) or phosphate-buffered saline (PBS) for 30 minutes at 37°C. Cell viability, phenotype (by flow cytometry), inhibition of T-cell proliferative responses and differentiation into lung epithelium-like cells (assessed with immunohistochemical staining of surfactant protein (SP)-A) were investigated. RESULTS: CONCLUSION:
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Authors | Courtney A McDonald, Jacqueline M Melville, Graeme R Polglase, Graham Jenkin, Timothy J M Moss |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 5
Issue 5
Pg. 107
(Sep 04 2014)
ISSN: 1757-6512 [Electronic] England |
PMID | 25189170
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biological Products
- Phospholipids
- Pulmonary Surfactants
- poractant alfa
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Topics |
- Amnion
(cytology, drug effects, metabolism)
- Animals
- Apoptosis
(drug effects)
- Biological Products
(pharmacology)
- Cell Culture Techniques
(methods)
- Cell Survival
(drug effects)
- Epithelial Cells
(cytology, drug effects, metabolism)
- Female
- Flow Cytometry
- Humans
- Infant, Newborn
- Mice
- Mice, Inbred C57BL
- Phenotype
- Phospholipids
(pharmacology)
- Pregnancy
- Pulmonary Surfactants
(metabolism, pharmacology)
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