The purpose of this study was to investigate the underlying mechanism(s) of the total
alkaloids (TA) from Mahonia bealei in treating pyloric
ligation-induced
gastric ulcers in rats. Animals were sacrificed after 19 h of the
ligation. Gastric acid, peptic activities,
mucin levels, H(+)/K(+)-
ATPase activities and the
gastrin level were analyzed. To improve the accuracy of the observations,
IPP 6.0 software was introduced to measure the area of
ulcer. TA (18.56 mg/kg/day, i.g.) showed an antiulcer effect by significantly decreasing the
gastric ulcer areas (11.28 mm(2)) compared with model group (26.36 mm(2)). The TA
ulcer inhibition ratio was 57.2%, compared with the effect of the positive control,
omeprazole (62.96%). The results also showed that TA had a significant effect in inhibiting the release of H(+)/K(+)-
ATPase, reducing the content of
gastrin and decreasing gastric acidity on experimental animals. However, the TA had no significant effects on gastric mucus secretion and
pepsin activity. Data indicated that TA had
gastric ulcer protective effects by modulating the H(+)/K(+)-
ATPase activity and
gastrin level. TA has a potential to be developed as a pharmacological agent for the treatment of
gastric ulcers.