Abstract | BACKGROUND AND OBJECTIVE: Deficiency of vitamin D could be a major cause of colon cancer as suggested as early as 1980 by Drs. Cedric and Frank Garland of the University of California and has recently been underscored by a large European case control study. Whether vitamin D deficiency is because of inadequate intake (food and sunshine) or because of vitamin D metabolism disorder in the patient body is unknown. A proteomics approach to identify protein pathways associated with vitamin D transportation and metabolism pathways will help us understand better the pathology of the colon cancer. METHODS: Lysates of colon adenocarcinoma tissues and their matched healthy tissues, from seven colon cancer patients, have been evaluated by quantitative proteomics and bioinformatics analysis to determine protein expression profiles. Unsupervised hierarchical clustering and principle component analysis (PCA) were utilized for protein expression profiling and biomarker identification, while the reporter ion ratios from tandem-mass-tagging (TMT) labeled peptides were used for quantification. Ingenuity Pathway Analysis (IPA) was used to analyze protein pathways. RESULTS AND CONCLUSION:
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Authors | Hui Tang, Saied Mirshahidi, Maheswari Senthil, Kevork Kazanjian, Chien-Shing Chen, Kangling Zhang |
Journal | Cancer biomarkers : section A of Disease markers
(Cancer Biomark)
Vol. 14
Issue 5
Pg. 313-24
( 2014)
ISSN: 1875-8592 [Electronic] Netherlands |
PMID | 25171473
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Retinoid X Receptors
- Vitamin D
- Vitamin E
- Heme
- Iron
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Topics |
- Acute-Phase Reaction
(genetics)
- Adenocarcinoma
(genetics)
- Aged
- Biomarkers, Tumor
(genetics)
- Case-Control Studies
- Colonic Neoplasms
(genetics)
- Computational Biology
(methods)
- Down-Regulation
(genetics)
- Female
- Gene Expression Profiling
(methods)
- Heme
(metabolism)
- Humans
- Iron
(metabolism)
- Lipid Metabolism
(genetics)
- Male
- Middle Aged
- Proteomics
(methods)
- Retinoid X Receptors
(genetics)
- Vitamin D
(metabolism)
- Vitamin E
(metabolism)
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