Abstract | BACKGROUND AND AIM: METHODS: Cultured hepatocytes were treated with lipopolysaccharide (LPS) in the presence or absence of UA. The reactive oxygen species (ROS) level, protein levels of IκBα, iNOS and Cox-2, and NF-κB activation were detected, respectively. C57/BL6 and AMP-activated protein kinase (AMPK)α2(-/-) mice were subjected to BDL for 14 days. UA was administered by gavage. The markers of liver function and oxidative stress, and liver histopathology were analyzed after treatment. RESULTS: Treatment of hepatocytes with UA dose-dependently activates AMPK, which is abolished by silence of liver kinase B1 (LKB1). LPS significantly increased ROS productions, apoptosis, NF-κB activation, and expressions of iNOS and Cox-2 in cultured hepatocytes. All these effects were blocked by co-incubation with UA. Importantly, silence of LKB1, AMPK, or iNOS/Cox-2 by small interference RNA transfection reversed UA-induced effects in cultured cells. In an animal study, 14-day BDL induced liver fibrosis and liver injury, accompanied with increased oxidative stress and protein expressions of iNOS and Cox-2 in liver. Treatment of UA significantly attenuated the BDL-induced detrimental effects in wild-type mice but not in AMPKα2(-/-) mice. CONCLUSION: UA via LKB1-AMPK signaling offers protective effects on BDL-induced liver injury in mice, which may be related to inhibition of oxidative stress.
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Authors | Yongbin Yang, Zhanxue Zhao, Yuanjun Liu, Xianjiang Kang, Haisong Zhang, Ming Meng |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 30
Issue 3
Pg. 609-18
(Mar 2015)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 25168399
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd. |
Chemical References |
- Lipopolysaccharides
- Reactive Oxygen Species
- Triterpenes
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
- Ptgs2 protein, mouse
- Cyclooxygenase 2
- Protein Serine-Threonine Kinases
- Stk11 protein, mouse
- AMP-Activated Protein Kinases
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Topics |
- AMP-Activated Protein Kinases
(physiology)
- Animals
- Cells, Cultured
- Cyclooxygenase 2
(metabolism)
- Depression, Chemical
- Dose-Response Relationship, Drug
- Hepatocytes
(enzymology, metabolism)
- Lipopolysaccharides
(adverse effects, antagonists & inhibitors)
- Liver Cirrhosis
(drug therapy, etiology)
- Liver Diseases
(drug therapy, etiology)
- Mice, Inbred C57BL
- Nitric Oxide Synthase Type II
(metabolism)
- Oxidative Stress
(drug effects)
- Protein Serine-Threonine Kinases
(physiology)
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
(drug effects, genetics, physiology)
- Triterpenes
(pharmacology, therapeutic use)
- Ursolic Acid
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