Expression profiling of microRNAs in hippocampus of rats following traumatic brain injury.

Abstract

The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.

• Authors: Ting-Yi Sun, Xiao-Rui Chen, Zi-Long Liu, Li-Li Zhao, Yong-Xiang Jiang, Guo-Qiang Qu, Rong-Shuai Wang, Si-Zhe Huang, Liang Liu
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 34 Issue 4 Pg. 548-553 (Aug 2014) ISSN: 1672-0733 [Print] China
• PMID: 25135725 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• MIRN142 microRNA, rat
• MIRN221 microRNA, rat
• MicroRNAs

Topics

• Animals
• Biomarkers (metabolism)
• Brain Injuries (metabolism, pathology)
• Female
• Forensic Genetics
• Gene Expression Profiling
• Gene Expression Regulation
• Hippocampus (metabolism, pathology)
• Male
• MicroRNAs (biosynthesis)
• Rats
• Rats, Sprague-Dawley