Silencing of phosphoinositide-specific phospholipase C ε remodulates the expression of the phosphoinositide signal transduction pathway in human osteosarcoma cell lines.

Abstract

BACKGROUND:

Ezrin, a member of the ezrin-radixin-moesin family, is involved in the metastatic spread of osteosarcoma. Ezrin binds phosphatydil inositol-4,5-bisphosphate (PIP2), a crucial molecule of the phosphoinositide signal transduction pathway. PIP2 levels are regulated by phosphoinositide-specific phospholipase C (PI-PLC) enzymes. PI-PLCε isoform, a well-characterized direct effector of rat sarcoma (RAS), is at a unique convergence point for the broad range of signaling pathways that promote RAS GTPase-mediated signalling.

MATERIALS AND METHODS:

By using molecular biology methods and microscopic analyses, we analyzed the expression of ezrin and PLC genes after silencing of PLCE (OMIM *608414) in 143B and Hs888 cell lines.

RESULTS:

The growth rate of the cells was slowed, and the expression of ezrin, PLCB1, PLCG2 and PLCD4 was significantly modified. Ezrin displacement from the plasma membrane was observed.

CONCLUSION:

The present results corroborate the hypothesis that ezrin and the PI signal transduction system are involved in a common network.

• Authors: Vincenza Rita Lo Vasco, Martina Leopizzi, Daniela Stoppoloni, Carlo Della Rocca
• Journal: Anticancer research (Anticancer Res) Vol. 34 Issue 8 Pg. 4069-75 (Aug 2014) ISSN: 1791-7530 [Electronic] Greece
• PMID: 25075031 (Publication Type: Journal Article)
• Copyright: Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• Cytoskeletal Proteins
• Phosphatidylinositols
• ezrin
• PLCB1 protein, human
• Phosphoinositide Phospholipase C
• Phospholipase C beta
• phospholipase C epsilon

Topics

• Bone Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Cytoskeletal Proteins (analysis, genetics)
• Gene Expression Regulation, Neoplastic
• Humans
• Osteosarcoma (metabolism, pathology)
• Phosphatidylinositols (physiology)
• Phosphoinositide Phospholipase C (analysis, genetics, physiology)
• Phospholipase C beta (genetics)
• Real-Time Polymerase Chain Reaction
• Signal Transduction (physiology)