Abstract |
The growth and metastasis of solid tumors depends on angiogenesis. Anti-angiogenesis therapy may represent a promising therapeutic option. Vasostatin, the N-terminal domain of calreticulin, is a very potent endogenous inhibitor of angiogenesis and tumor growth. In this study, we attempted to investigate whether plasmid-encoding vasostatin complexed with cationic liposome could suppress the growth and metastasis of hepatocellular carcinoma in vivo and discover its possible mechanism of action. Apoptosis induction of pSecTag2B-vasostatin plasmid on murine endothelial cells (MS1) was examined by flow cytometric analysis in vitro. Nude mice bearing HCCLM3 tumor received pSecTag2B-vasostatin, pSecTag2B-Null, and 0.9 % NaCl solution, respectively. Tumor net weight was measured and survival time was observed. Microvessel density within tumor tissues was determined by CD31 immunohistochemistry. H&E staining of lungs and TUNEL assay of primary tumor tissues were also conducted. The results displayed that pSecTag2B-vasostatin could inhibit the growth and metastasis of hepatocellular carcinoma xenografts and prolong survival time compared with the controls in vivo. Moreover, histologic analysis revealed that pSecTag2B-vasostatin treatment increased apoptosis and inhibited angiogenesis. The present data may be of importance to the further exploration of this new anti-angiogenesis approach in the treatment of hepatocellular cancer.
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Authors | Xing-Chen Peng, Ming Wang, Xu-Xia Chen, Jing Liu, Gui-Hua Xiao, Hong-Li Liao |
Journal | Molecular and cellular biochemistry
(Mol Cell Biochem)
Vol. 395
Issue 1-2
Pg. 265-72
(Oct 2014)
ISSN: 1573-4919 [Electronic] Netherlands |
PMID | 24997628
(Publication Type: Journal Article)
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Chemical References |
- Calreticulin
- Liposomes
- Peptide Fragments
- vasostatin
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Topics |
- Animals
- Calreticulin
(genetics, metabolism, pharmacology)
- Carcinoma, Hepatocellular
(pathology, therapy)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Female
- Genetic Therapy
- Genetic Vectors
(administration & dosage)
- Humans
- Liposomes
(administration & dosage)
- Liver Neoplasms
(pathology, therapy)
- Mice
- Neoplasm Metastasis
- Neoplasms, Experimental
- Peptide Fragments
(genetics, metabolism, pharmacology)
- Plasmids
(genetics)
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