Effect of detorsion alone and in combination with enoxaparin therapy on ovarian reserve and serum antimüllerian hormone levels in a rat ovarian torsion model.

Abstract

OBJECTIVE:

To investigate the effect of enoxaparin on ovarian reserve and serum antimüllerian hormone (AMH) levels in a rat ovarian torsion model.

DESIGN:

Experimental study.

SETTING:

Experimental surgery laboratory in a training and research hospital.

ANIMAL(S):

Fourteen female Wistar Hannover rats.

INTERVENTION(S):

1) Control group received no special treatment other than abdominal exposure; 2) detorsion-only group received bilateral adnexal torsion (3-hour ischemia), and then after 3-hour torsion period, detorsion (reperfusion) was performed; and 3) detorsion-enoxaparin group received 0.5 mg/kg enoxaparin subcutaneously 2 hours before the same surgery as the detorsion-only group and a second 0.5 mg/kg dose of enoxaparin 24 hours after the first surgeries. Apart from the surgeries, preoperative and postoperative 1-mL blood samples were drawn from the right jugular vein of each rat.

MAIN OUTCOME MEASURE(S):

Preoperative and postoperative serum AMH levels, histopathologic damage scores, and follicle counts in the ovarian tissue of the rats.

RESULT(S):

Vascular congestion and hemorrhage scores were higher in the detorsion-enoxaparin group than in the detorsion-only and control groups. The number of small antral follicles was smaller in the detorsion-only group than in the control group. The difference in the pre- and postoperative AMH levels was higher in the detorsion-only group than in the control and detorsion-enoxaparin groups.

CONCLUSION(S):

The combination of enoxaparin therapy with conventional ovarian detorsion is more effective in protecting the ovarian reserve than detorsion alone.

• Authors: Cihan Kaya, Hürriyet Turgut, Hüseyin Cengiz, Ayşenur Turan, Murat Ekin, Levent Yaşar
• Journal: Fertility and sterility (Fertil Steril) Vol. 102 Issue 3 Pg. 878-884.e1 (Sep 2014) ISSN: 1556-5653 [Electronic] United States
• PMID: 24996496 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Chemical References

• Enoxaparin
• Anti-Mullerian Hormone

Topics

• Animals
• Anti-Mullerian Hormone (blood)
• Cell Count
• Combined Modality Therapy
• Disease Models, Animal
• Enoxaparin (therapeutic use)
• Fallopian Tube Diseases (blood, drug therapy, surgery)
• Female
• Gynecologic Surgical Procedures (methods)
• Ovary (cytology, drug effects)
• Rats
• Rats, Wistar
• Reperfusion Injury (prevention & control)
• Torsion Abnormality (blood, drug therapy, surgery)