Ferrocenylnaphthalene diimide-based electrochemical detection of aberrant methylation in hTERT gene.

Abstract

Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene (hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip.

• Authors: Shinobu Sato, Toshiro Saeki, Tomoki Tanaka, Yusuke Kanezaki, Mana Hayakawa, Kazuya Haraguchi, Masaaki Kodama, Tatsuji Nishihara, Kazuhiro Tominaga, Shigeori Takenaka
• Journal: Applied biochemistry and biotechnology (Appl Biochem Biotechnol) Vol. 174 Issue 3 Pg. 869-79 (Oct 2014) ISSN: 1559-0291 [Electronic] United States
• PMID: 24993489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Ferrous Compounds
• Imides
• ferrocenylnaphthalene diimide
• TERT protein, human
• Telomerase

Topics

• Biomarkers, Tumor (genetics, isolation & purification)
• Biosensing Techniques
• CpG Islands (genetics)
• DNA Methylation (genetics)
• Electrochemistry
• Electrodes
• Ferrous Compounds (chemistry)
• Humans
• Imides (chemistry)
• Neoplasms (diagnosis, genetics)
• Telomerase (genetics, isolation & purification)