Keratinocyte growth factor-2 is protective in lipopolysaccharide-induced acute lung injury in rats.

Abstract

Keratinocyte growth factor-2 (KGF-2) plays a key role in lung development, but its role in acute lung injury has not been well characterized. Lipopolysaccharide instillation caused acute lung injury, which significantly elevated lung wet-to-dry weight ratio, protein and neutrophils in bronchoalveolar lavage fluid (BALF), inhibited surfactant protein A and C expression in lung tissue, and increased pathological injury. Pretreatment with KGF-2 improved the above lung injury parameters, partially restored surfactant protein A and C expression, and KGF-2 given 2-3 days before LPS challenge showed maximum lung injury improvement. Pretreatment with KGF-2 also markedly reduced the levels of TNF-α, MIP-2, IL-1β and IL-6 in BALF and the levels of IL-1β and IL-6 in lung tissue. Histological analysis showed there was increased proliferation of alveolar type II epithelial cells in lung parenchyma, which reached maximal 2 days after KGF-2 instillation. Intratracheal administration of KGF-2 attenuates lung injury induced by LPS, suggesting KGF-2 may be potent in the intervention of acute lung injury.

• Authors: Lin Tong, Jing Bi, Xiaodan Zhu, Guifang Wang, Jie Liu, Linyi Rong, Qin Wang, Nuo Xu, Ming Zhong, Duming Zhu, Yuanlin Song, Chunxue Bai
• Journal: Respiratory physiology & neurobiology (Respir Physiol Neurobiol) Vol. 201 Pg. 7-14 (Sep 15 2014) ISSN: 1878-1519 [Electronic] Netherlands
• PMID: 24973472 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Cytokines
• Fibroblast Growth Factor 10
• Ki-67 Antigen
• Lipopolysaccharides
• Peptides
• RNA, Messenger
• Sftpc protein, rat

Topics

• Acute Lung Injury (chemically induced, pathology, prevention & control)
• Animals
• Bronchoalveolar Lavage Fluid (chemistry)
• Cytokines (genetics, metabolism)
• Disease Models, Animal
• Fibroblast Growth Factor 10 (therapeutic use)
• Ki-67 Antigen (metabolism)
• Lipopolysaccharides (toxicity)
• Lung (drug effects, pathology, ultrastructure)
• Male
• Neutrophils (pathology)
• Peptides (metabolism)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Time Factors