Abstract |
Catechins may influence both desirable and undesirable effects of many drugs. In this study, the in vitro effect of (+)- catechin, (-)- epicatechin, (-)- epigallocatechin, (-)-epicatechin gallate, and (-)- epigallocatechin gallate (EGCG) on the efficacy of anticancer drug doxorubicin (DOX) was studied in HCT-8 cancer cells. Rat hepatocytes were used to study the influence of EGCG on DOX hepatotoxicity. Cell proliferation and viability were studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5- diphenyl tetrazolium bromide and neutral red uptake test assays. Formation of reactive oxygen species (ROS) was determined using the dichlorofluorescein assay. All of the studied catechins (1-25 μmol L(-1)) had no effect on the proliferation of intestinal cancer cells and did not affect the antiproliferative effect of DOX (1-8 μmol L(-1)) in these cells. Moreover, EGCG at 25 μmol L(-1) increased the viability of isolated hepatocytes and significantly protected these cells against DOX-induced toxicity and ROS production. Consumption of EGCG during DOX therapy seems to be safe and beneficial, since EGCG does not decrease DOX anticancer efficacy and could ameliorate DOX hepatotoxicity.
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Authors | Petra Rudolfová, Veronika Hanušová, Lenka Skálová, Hana Bártíková, Petra Matoušková, Iva Boušová |
Journal | Acta pharmaceutica (Zagreb, Croatia)
(Acta Pharm)
Vol. 64
Issue 2
Pg. 199-209
(Jun 2014)
ISSN: 1846-9558 [Electronic] Poland |
PMID | 24914720
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Reactive Oxygen Species
- Doxorubicin
- Catechin
- epigallocatechin gallate
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Topics |
- Animals
- Antineoplastic Agents
(adverse effects, pharmacology)
- Catechin
(analogs & derivatives, pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Doxorubicin
(adverse effects, pharmacology)
- Hepatocytes
(drug effects)
- Humans
- Male
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
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