MicroRNA expression patterns in adrenocortical carcinoma variants and clinical pathologic correlations.

Abstract

Several microRNAs (miRNAs) were shown to be deregulated in adrenocortical carcinoma (ACC) as compared with adenoma, but a detailed assessment of their expression in its histologic variants and correlation with clinicopathologic characteristics has not been performed, so far. Our aim was to assess the expression of 5 selected miRNAs (IGF2 gene-related miR-483-3p and 5p and hypoxia-induced miR-210, miR-195, and miR-1974) in a series of 51 ACCs (35 classical, 6 myxoid, and 10 oncocytic) as compared with clinical and pathologic features and immunohistochemical expression of prognostic markers, including steroidogenic factor 1, p53, β-catenin, and glucose transporter 1. Oncocytic carcinomas had a reduced expression of miR-483-3p (P = .0325), miR-483-5p (P = .0175), and miR-210 (P = .0366), as compared with other histotypes. Overexpression of miR-210 was associated with the presence of necrosis (P = .0035), high Ki-67 index (P = .0013), and high glucose transporter 1 expression (P = .0043), whereas an inverse correlation with mitotic rate was observed in cases with high miR-493-3p (P = .0191) and miR-1974 (P = .0017) expression. High miR-1974 was also associated with low Ki-67 (P = .0312) and European Network for the Study of Adrenal Tumors stage (P = .0082) and negative p53 (P = .0013). At univariate analysis myxoid/classic histotype (P = .026), high miR-210 (P = .0465), high steroidogenic factor 1 protein (P = .0017), high Ki-67 (P = .0066), and high mitotic index (P = .0006) were significantly associated the shorter overall survival, the latter being the sole independent prognostic factor at multivariate analysis (P = .017). In conclusion, (a) miR-483-3p, miR-483-5p, and miR-210 are differentially expressed in ACC variants, and (b) high miR-210 is associated with clinicopathologic parameters of aggressiveness and a poor prognosis.

• Authors: Eleonora Duregon, Ida Rapa, Arianna Votta, Jessica Giorcelli, Fulvia Daffara, Massimo Terzolo, Giorgio V Scagliotti, Marco Volante, Mauro Papotti
• Journal: Human pathology (Hum Pathol) Vol. 45 Issue 8 Pg. 1555-62 (Aug 2014) ISSN: 1532-8392 [Electronic] United States
• PMID: 24890943 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• MIRN195 microRNA, human
• MIRN210 microRNA, human
• MIRN483 microRNA, human
• MicroRNAs

Topics

• Adrenal Cortex Neoplasms (genetics, metabolism, pathology)
• Adrenocortical Carcinoma (genetics, metabolism, pathology)
• Adult
• Aged
• Female
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Neoplasm Staging
• Prognosis
• Young Adult