Human selenium binding protein-1 (hSP56) is a negative regulator of HIF-1α and suppresses the malignant characteristics of prostate cancer cells.

Abstract

In the present study, we demonstrate that ectopic expression of 56-kDa human selenium binding protein-1 (hSP56) in PC-3 cells that do not normally express hSP56 results in a marked inhibition of cell growth in vitro and in vivo. Down-regulation of hSP56 in LNCaP cells that normally express hSP56 results in enhanced anchorage-independent growth. PC-3 cells expressing hSP56 exhibit a significant reduction of hypoxia inducible protein (HIF)-1α protein levels under hypoxic conditions without altering HIF-1α mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays a critical role in prostate cells by mechanisms including negative regulation of HIF-1α, thus identifying hSP56 as a candidate anti-oncogene product.

• Authors: Jee-Yeong Jeong, Jin-Rong Zhou, Chong Gao, Laurie Feldman, Arthur J Sytkowski
• Journal: BMB reports (BMB Rep) Vol. 47 Issue 7 Pg. 411-6 (Jul 2014) ISSN: 1976-670X [Electronic] Korea (South)
• PMID: 24874852 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Hypoxia-Inducible Factor 1, alpha Subunit
• RNA, Messenger
• SELENBP1 protein, human
• Selenium-Binding Proteins
• USP20 protein, human
• USP33 protein, human
• Ubiquitin Thiolesterase

Topics

• Animals
• COS Cells
• Cell Line, Tumor
• Cell Transformation, Neoplastic
• Chlorocebus aethiops
• Down-Regulation
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (antagonists & inhibitors, genetics, metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Mice, SCID
• Prostatic Neoplasms (metabolism, pathology)
• Protein Binding
• RNA Interference
• RNA, Messenger (metabolism)
• Selenium-Binding Proteins (metabolism)
• Transplantation, Heterologous
• Ubiquitin Thiolesterase (metabolism)