GATA4 immunolocalization in breast carcinoma as a potent prognostic predictor.

Abstract

Transcriptional GATA factors are known lineage selector genes and regulate a variety of biological processes including specification and differentiation of tissues. In the present study, we examined expression profiles of six GATA factor genes in invasive ductal carcinomas (IDC) of the breast using microarray analysis (n = 20) and found that GATA4 expression was closely correlated with recurrence in patients. Because the significance of GATA4 has remained largely unknown in breast carcinoma, we further immunolocalized GATA4 in ductal carcinoma in situ (DCIS) of the breast (n = 48) and IDC (n = 163). GATA4 immunoreactivity was detected in the nuclei of carcinoma cells and was positive in 27% of DCIS and 31% of IDC cases. GATA4 status was significantly associated with nuclear grade and van Nuys classification in DCIS and was positively associated with distant metastasis, histological grade and HER2 status, but negatively correlated with progesterone receptor labeling index in IDC. Subsequent multivariate analysis demonstrated that GATA4 status was an independent prognostic factor for both disease-free and breast cancer-specific survival of IDC patients. All of these results indicate that GATA4 plays important roles in the progression of breast carcinoma from an early stage and that immunohistochemical GATA4 status is considered a potent prognostic factor in human breast cancer patients.

• Authors: Kiyoshi Takagi, Takashi Moriguchi, Yasuhiro Miki, Yasuhiro Nakamura, Mika Watanabe, Takanori Ishida, Masayuki Yamamoto, Hironobu Sasano, Takashi Suzuki
• Journal: Cancer science (Cancer Sci) Vol. 105 Issue 5 Pg. 600-7 (May 2014) ISSN: 1349-7006 [Electronic] England
• PMID: 24862985 (Publication Type: Journal Article)
• Copyright: © 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

Chemical References

• GATA4 Transcription Factor
• GATA4 protein, human
• Receptors, Progesterone
• ERBB2 protein, human
• Receptor, ErbB-2

Topics

• Adult
• Aged
• Breast Neoplasms (genetics, metabolism, pathology)
• Carcinoma, Ductal, Breast (pathology)
• Disease-Free Survival
• Female
• GATA4 Transcription Factor (genetics, metabolism)
• Gene Expression
• Humans
• Immunohistochemistry
• Laser Capture Microdissection
• Middle Aged
• Neoplasm Recurrence, Local (genetics, pathology)
• Receptor, ErbB-2 (metabolism)
• Receptors, Progesterone (metabolism)