VE-cadherin Y685F knock-in mouse is sensitive to vascular permeability in recurrent angiogenic organs.

Abstract

Covalent modifications such as tyrosine phosphorylation are associated with the breakdown of endothelial cell junctions and increased vascular permeability. We previously showed that vascular endothelial (VE)-cadherin was tyrosine phosphorylated in vivo in the mouse reproductive tract and that Y685 was a target site for Src in response to vascular endothelial growth factor in vitro. In the present study, we aimed to understand the implication of VE-cadherin phosphorylation at site Y685 in cyclic angiogenic organs. To achieve this aim, we generated a knock-in mouse carrying a tyrosine-to-phenylalanine point mutation of VE-cadherin Y685 (VE-Y685F). Although homozygous VE-Y685F mice were viable and fertile, the nulliparous knock-in female mice exhibited enlarged uteri with edema. This phenotype was observed in 30% of females between 4 to 14 mo old. Histological examination of longitudinal sections of the VE-Y685F uterus showed an extensive disorganization of myometrium and endometrium with highly edematous uterine glands, numerous areas with sparse cells, and increased accumulation of collagen fibers around blood vessels, indicating a fibrotic state. Analysis of cross section of ovaries showed the appearance of spontaneous cysts, which suggested increased vascular hyperpermeability. Electron microscopy analysis of capillaries in the ovary showed a slight but significant increase in the gap size between two adjacent endothelial cell membranes in the junctions of VE-Y685F mice (wild-type, 11.5 ± 0.3, n = 78; and VE-Y685F, 12.48 ± 0.3, n = 65; P = 0.045), as well as collagen fiber accumulation around capillaries. Miles assay revealed that either basal or vascular endothelial growth factor-stimulated permeability in the skin was increased in VE-Y685F mice. Since edema and fibrotic appearance have been identified as hallmarks of initial increased vascular permeability, we conclude that the site Y685 in VE-cadherin is involved in the physiological regulation of capillary permeability. Furthermore, this knock-in mouse model is of potential interest for further studies of diseases that are associated with abnormal vascular permeability.

• Authors: Adama Sidibé, Helena Polena, Karin Pernet-Gallay, Jeremy Razanajatovo, Tiphaine Mannic, Nicolas Chaumontel, Soumalamaya Bama, Irène Maréchal, Philippe Huber, Danielle Gulino-Debrac, Laurence Bouillet, Isabelle Vilgrain
• Journal: American journal of physiology. Heart and circulatory physiology (Am J Physiol Heart Circ Physiol) Vol. 307 Issue 3 Pg. H455-63 (Aug 01 2014) ISSN: 1522-1539 [Electronic] United States
• PMID: 24858856 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 the American Physiological Society.

Chemical References

• Antigens, CD
• Cadherins
• cadherin 5
• Tyrosine

Topics

• Animals
• Antigens, CD (genetics, metabolism)
• Cadherins (genetics, metabolism)
• Capillaries (metabolism, physiopathology, ultrastructure)
• Capillary Permeability
• Edema (metabolism, pathology, physiopathology)
• Female
• Gene Knock-In Techniques
• Genotype
• Homozygote
• Mice, Inbred C57BL
• Mice, Transgenic
• Neovascularization, Physiologic
• Ovary (blood supply)
• Phenotype
• Phosphorylation
• Tyrosine
• Uterus (blood supply)