Up-regulation of CHAF1A, a poor prognostic factor, facilitates cell proliferation of colon cancer.

Abstract

Deregulation of chromatin assembly factor 1, p150 subunit A (CHAF1A) has recently been reported to be involved in the development of some cancer types. In this study, we identified that the frequency of positive CHAF1A staining in primary tumor mucosa (45.8%, 93 of 203 samples) was significantly elevated compared to that in paired normal mucosa (18.7%, 38 of 203 samples). The increased expression was strongly associated with cancer stage, tumor invasion, and histological grade. The five-year survival rate of patients with CHAF1A-positive tumors was remarkably lower than that of patients with CHAF1A-negative tumors. Colon cancer cells with CHAF1A knockdown exhibited decreased cell growth index, reduction in colony formation ability, elevated cell apoptosis rate as well as impaired colon tumorigenicity in nude mice. Hence, CHAF1A upregulation functions as a poor prognostic indicator of colon cancer, potentially contributing to its progression by mediating cancer cell proliferation.

• Authors: Zehua Wu, Feifei Cui, Fudong Yu, Xiao Peng, Tao Jiang, Dawei Chen, Su Lu, Huamei Tang, Zhihai Peng
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 449 Issue 2 Pg. 208-15 (Jun 27 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 24845563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers, Tumor
• CNOT8 protein, human
• Chromatin Assembly Factor-1
• RNA, Messenger
• RNA, Neoplasm
• Transcription Factors

Topics

• Adult
• Aged
• Aged, 80 and over
• Animals
• Biomarkers, Tumor (genetics, metabolism)
• Cell Line, Tumor
• Cell Proliferation
• Chromatin Assembly Factor-1 (antagonists & inhibitors, genetics, metabolism)
• Colonic Neoplasms (genetics, metabolism, pathology)
• Female
• Gene Knockdown Techniques
• Heterografts
• Humans
• Kaplan-Meier Estimate
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Neoplasm Invasiveness
• Neoplasm Staging
• Prognosis
• RNA, Messenger (genetics, metabolism)
• RNA, Neoplasm (genetics, metabolism)
• Transcription Factors
• Tumor Stem Cell Assay
• Up-Regulation
• Young Adult