Abstract | BACKGROUND:
Airway remodeling is implicated in irreversible airflow limitation of refractory asthma, which includes increased smooth muscle mass and subepithelial fibrosis. Activated fibroblasts acquire contractile phenotype to participate in tissue contraction and structural alteration of extracellular matrices. Histamine is a potent mediator of allergic inflammation, substantially involved in asthmatic pathophysiology. OBJECTIVE: METHODS: RESULTS:
Histamine induced contraction of collagen gels embedded with human lung fibroblasts, in a time-dependent manner, and at the concentration more than 10(-6) M, both in four primary cultured adult lung fibroblasts and three fetal lung fibroblast cell lines. This effect was attenuated by H1 receptor antagonist, whereas those for H2 to H4 receptors failed to show an inhibitory effect. Furthermore, IP3 receptor-mediated Ca(2+) mobilization was implicated in histamine's action on collagen gel contraction. CONCLUSIONS:
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Authors | Masafumi Horie, Akira Saito, Yasuhiro Yamauchi, Yu Mikami, Makiko Sakamoto, Taisuke Jo, Jun Nakajima, Hajime Takizawa, Takahide Nagase, Tadashi Kohyama |
Journal | Experimental lung research
(Exp Lung Res)
Vol. 40
Issue 5
Pg. 222-36
(Jun 2014)
ISSN: 1521-0499 [Electronic] England |
PMID | 24809793
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dipeptides
- N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide
- Receptors, Histamine H1
- Histamine
- Collagen
- Matrix Metalloproteinase 2
- Calcium
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Topics |
- Airway Remodeling
(drug effects)
- Calcium
(metabolism)
- Cells, Cultured
- Collagen
(physiology)
- Dipeptides
(pharmacology)
- Fibroblasts
(drug effects, physiology)
- Histamine
(pharmacology)
- Humans
- Matrix Metalloproteinase 2
(analysis)
- Receptors, Histamine H1
(physiology)
- Signal Transduction
(physiology)
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