Acute sleep deprivation enhances post-infection sleep and promotes survival during bacterial infection in Drosophila.

Abstract

STUDY OBJECTIVES:

Sleep is known to increase as an acute response to infection. However, the function of this behavioral response in host defense is not well understood. To address this problem, we evaluated the effect of acute sleep deprivation on post-infection sleep and immune function in Drosophila.

SETTING:

Laboratory.

PARTICIPANTS:

Drosophila melanogaster.

METHODS AND RESULTS:

Flies were subjected to sleep deprivation before (early DEP) or after (late DEP) bacterial infection. Relative to a non-deprived control, flies subjected to early DEP had enhanced sleep after infection as well as increased bacterial clearance and survival outcome. Flies subjected to late DEP experienced enhanced sleep following the deprivation period, and showed a modest improvement in survival outcome. Continuous DEP (early and late DEP) throughout infection also enhanced sleep later during infection and improved survival. However, improved survival in flies subjected to late or continuous DEP did not occur until after flies had experienced sleep. During infection, both early and late DEP enhanced NFκB transcriptional activity as measured by a luciferase reporter (κB-luc) in living flies. Early DEP also increased NFκB activity prior to infection. Flies that were deficient in expression of either the Relish or Dif NFκB transcription factors showed normal responses to early DEP. However, the effect of early DEP on post-infection sleep and survival was abolished in double mutants, which indicates that Relish and Dif have redundant roles in this process.

CONCLUSIONS:

Acute sleep deprivation elevated NFκB-dependent activity, increased post-infection sleep, and improved survival during bacterial infection.

• Authors: Tzu-Hsing Kuo, Julie A Williams
• Journal: Sleep (Sleep) Vol. 37 Issue 5 Pg. 859-69 (May 01 2014) ISSN: 1550-9109 [Electronic] United States
• PMID: 24790264 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA-Binding Proteins
• Dif protein, Drosophila
• Drosophila Proteins
• NF-kappa B
• Rel protein, Drosophila
• Transcription Factors

Topics

• Animals
• DNA-Binding Proteins (genetics, metabolism)
• Drosophila Proteins (genetics, metabolism)
• Drosophila melanogaster (genetics, immunology, microbiology, physiology)
• Female
• NF-kappa B (metabolism)
• Sleep (genetics, immunology, physiology)
• Sleep Deprivation (immunology, physiopathology)
• Survival Analysis
• Transcription Factors (genetics, metabolism)