Serum paraoxonase-1 activity is more closely related to HDL particle concentration and large HDL particles than to HDL cholesterol in Type 2 diabetic and non-diabetic subjects.

Abstract

OBJECTIVES:

We determined relationships of the anti-oxidative enzyme, paraoxonase-1 (PON-1), with high density lipoprotein (HDL) subfractions, and tested whether these relationships are stronger than those with HDL cholesterol and apolipoprotein A-I (apoA-I) in subjects with and without type 2 diabetes mellitus (T2DM).

DESIGN AND METHODS:

Serum PON-1 (arylesterase activity) and HDL subfractions (nuclear magnetic resonance spectroscopy) were determined in 67 T2DM patients and in 56 non-diabetic subjects.

RESULTS:

PON-1 activity, HDL cholesterol and apoA-I were decreased in T2DM (all p<0.05). The HDL particle concentration was unaltered, but large HDL particles, medium HDL particles and HDL particle size were decreased, whereas small HDL particles were increased in T2DM (all p<0.05). PON-1 was more closely related to HDL cholesterol than to apoA-I (p=0.001). In turn, the positive relationship of PON-1 with the HDL particle concentration and with large HDL particles was stronger than that with HDL cholesterol (both p<0.01). The inverse relationship of PON-1 with T2DM was only modestly attenuated by HDL cholesterol or HDL particle characteristics.

CONCLUSIONS:

PON-1 activity is more closely related to the HDL particle concentration or large HDL particles than to HDL cholesterol. Impaired PON-1 activity in T2DM is not to a considerable extent explained by altered HDL subfraction levels.

• Authors: Robin P F Dullaart, James D Otvos, Richard W James
• Journal: Clinical biochemistry (Clin Biochem) Vol. 47 Issue 12 Pg. 1022-7 (Aug 2014) ISSN: 1873-2933 [Electronic] United States
• PMID: 24769273 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Lipoproteins, HDL
• Aryldialkylphosphatase
• PON1 protein, human

Topics

• Aged
• Aryldialkylphosphatase (blood)
• Case-Control Studies
• Diabetes Mellitus, Type 2 (blood, enzymology)
• Female
• Humans
• Lipoproteins, HDL (blood)
• Magnetic Resonance Spectroscopy
• Male
• Middle Aged