Combined elevation of microRNA-196a and microRNA-196b in sera predicts unfavorable prognosis in patients with osteosarcomas.

Abstract

AIM:

To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma.

METHODS:

Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients' sera.

RESULTS:

Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p<0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p<0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p=0.008 and 0.01, respectively), positive metastasis (p=0.001 and 0.006, respectively) and recurrence (p=0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients.

CONCLUSION:

Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy.

• Authors: Chun Zhang, Cong Yao, Haopeng Li, Guoyu Wang, Xijng He
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 4 Pg. 6544-55 (Apr 17 2014) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 24747591 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• MIRN196 microRNA, human
• MicroRNAs

Topics

• Antineoplastic Agents (therapeutic use)
• Bone Neoplasms (diagnosis, drug therapy, genetics)
• Disease-Free Survival
• Female
• Humans
• Kaplan-Meier Estimate
• Male
• MicroRNAs (blood)
• Odds Ratio
• Osteosarcoma (diagnosis, drug therapy, genetics)
• Prognosis
• Recurrence
• Up-Regulation