Abstract |
As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with hPNPase(old-35) expression, were identified. The majority of these genes were associated with cell communication, cell cycle, and chromosomal organization gene ontology categories. For a number of these genes, the positive or negative correlations with hPNPase(old-35) expression were consistent with transcriptional data extracted from the TCGA (The Cancer Genome Atlas) expression datasets for colon adenocarcinoma ( COAD), skin cutaneous melanoma (SKCM), ovarian serous cyst adenocarcinoma (OV), and prostate adenocarcinoma (PRAD). Further analysis comparing the gene expression changes between Ad.hPNPase(old-35) infected HO-1 melanoma cells and HeLa cells overexpressing hPNPase(old-35) under the control of a doxycycline-inducible promoter, revealed global changes in genes involved in cell cycle and mitosis. Overall, this study provides further evidence that hPNPase(old-35) is associated with global changes in cell cycle-associated genes and identifies potential gene targets for future investigation.
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Authors | Upneet K Sokhi, Manny D Bacolod, Luni Emdad, Swadesh K Das, Catherine I Dumur, Michael F Miles, Devanand Sarkar, Paul B Fisher |
Journal | Journal of cellular physiology
(J Cell Physiol)
Vol. 229
Issue 12
Pg. 1952-62
(Dec 2014)
ISSN: 1097-4652 [Electronic] United States |
PMID | 24729470
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Wiley Periodicals, Inc. |
Chemical References |
- Exoribonucleases
- PNPT1 protein, human
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Topics |
- Apoptosis
(genetics)
- Cell Cycle
(genetics)
- Exoribonucleases
(biosynthesis, genetics, metabolism)
- Gene Expression Regulation, Neoplastic
(genetics)
- HeLa Cells
- Humans
- Melanoma
(genetics, pathology)
- Promoter Regions, Genetic
- Skin Neoplasms
- Melanoma, Cutaneous Malignant
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