Differential expression of microRNA-210 in gliomas of variable cell origin and correlation between increased expression levels and disease progression in astrocytic tumours.

Abstract

INTRODUCTION:

The microRNA, miR-210, is frequently associated with hypoxia induction, and an increase in its levels is often correlated with poor prognosis in many solid tumours. The present study examines the levels of miR-210 in glioma tumours of multiple origins to determine if an association can be established with disease progression.

MATERIAL AND METHODS:

Tissue samples were acquired from normal brain tissue, oligodendroglial tumours and astrocytic tumours. The astrocytic tumours were further divided by grade: diffuse astrocytomas (WHO grade II), anaplastic astrocytomas (WHO grade III), and glioblastoma (WHO grade IV). The expression of miR-210 was examined by real-time quantitative RT-PCR. The correlation of the expression of miR-210 and astrocytic tumour grade was analyzed by the Spearman correlation test.

RESULTS:

MiR-210 presents a differential expression depending on the origin of the glioma. Oligodendroglial tumours exhibit a significantly reduced level of miR-210 as compared with normal brain tissue. In contrast, astrocytic tumours demonstrate significantly increased levels of miR-210. Furthermore, the expression of miR-210 is positively correlated with the grade of astrocytic tumour, in the following order: grade IV > grade III > grade II > normal brain tissue (p < 0.05).

CONCLUSIONS:

MiR-210 levels can be potentially established as a biomarker for pathological diagnosis of malignant astrocytic tumour progression. In addition, the expression of miR-210 can be utilized as an additional identification measure of glioma tumour origin.

• Authors: Niansheng Lai, Hao Zhu, Yijun Chen, Shuai Zhang, Xudong Zhao, Yuchang Lin
• Journal: Folia neuropathologica (Folia Neuropathol) Vol. 52 Issue 1 Pg. 79-85 ( 2014) ISSN: 1509-572X [Electronic] Poland
• PMID: 24729345 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• MIRN210 microRNA, human
• MicroRNAs

Topics

• Adolescent
• Adult
• Aged
• Biomarkers, Tumor (genetics)
• Brain Neoplasms (genetics, pathology)
• Disease Progression
• Female
• Gene Expression Regulation, Neoplastic
• Glioma (genetics, pathology)
• Humans
• Male
• MicroRNAs (biosynthesis)
• Middle Aged
• Neoplasm Grading
• Real-Time Polymerase Chain Reaction
• Young Adult