Acute intermittent porphyria causes hepatic mitochondrial energetic failure in a mouse model.

Abstract

Acute intermittent porphyria (AIP), an inherited hepatic disorder, is due to a defect of hydroxymethylbilane synthase (HMBS), an enzyme involved in heme biosynthesis. AIP is characterized by recurrent, life-threatening attacks at least partly due to the increased hepatic production of 5-aminolaevulinic acid (ALA). Both the mitochondrial enzyme, ALA synthase (ALAS) 1, involved in the first step of heme biosynthesis, which is closely linked to mitochondrial bioenergetic pathways, and the promise of an ALAS1 siRNA hepatic therapy in humans, led us to investigate hepatic energetic metabolism in Hmbs KO mice treated with phenobarbital. The mitochondrial respiratory chain (RC) and the tricarboxylic acid (TCA) cycle were explored in the Hmbs(-/-) mouse model. RC and TCA cycle were significantly affected in comparison to controls in mice treated with phenobarbital with decreased activities of RC complexes I (-52%, (**)p<0.01), II (-50%, (**)p<0.01) and III (-55%, (*)p<0.05), and decreased activity of α-ketoglutarate dehydrogenase (-64%, (*)p<0.05), citrate synthase (-48%, (**)p<0.01) and succinate dehydrogenase (-53%, (*)p<0.05). Complex II-driven succinate respiration was also significantly affected. Most of these metabolic alterations were at least partially restored after the phenobarbital arrest and heme arginate administration. These results suggest a cataplerosis of the TCA cycle induced by phenobarbital, caused by the massive withdrawal of succinyl-CoA by ALAS induction, such that the TCA cycle is unable to supply the reduced cofactors to the RC. This profound and reversible impact of AIP on mitochondrial energetic metabolism offers new insights into the beneficial effect of heme, glucose and ALAS1 siRNA treatments by limiting the cataplerosis of TCA cycle.

• Authors: Chadi Homedan, Jihane Laafi, Caroline Schmitt, Naïg Gueguen, Thibaud Lefebvre, Zoubida Karim, Valérie Desquiret-Dumas, Céline Wetterwald, Jean-Charles Deybach, Laurent Gouya, Hervé Puy, Pascal Reynier, Yves Malthièry
• Journal: The international journal of biochemistry & cell biology (Int J Biochem Cell Biol) Vol. 51 Pg. 93-101 (Jun 2014) ISSN: 1878-5875 [Electronic] Netherlands
• PMID: 24727425 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Topics

• Animals
• Disease Models, Animal
• Energy Metabolism
• Female
• Liver Diseases (etiology, metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Mitochondria (metabolism)
• Oxidative Phosphorylation
• Porphyria, Acute Intermittent (complications, metabolism)