Abstract | BACKGROUND: MATERIALS AND METHODS: Thirteen Landrace-Large white pigs weighing 23 kg (range, 17-38 kg) underwent orthotopic liver transplantation. They were divided into two groups according to the size of the donor body: LFS and control (CTRL). After transplantation, the abdominal cavity of the recipient was kept open and portal venous flow (PVF) was measured after 1 h. The ratio of recipient PVF (PVFr) to donor PVF was used to establish correlations with ischemia and reperfusion parameters. Liver biopsies were taken 1 h after transplantation to assess ischemia and reperfusion and to quantify the gene expression of endothelial nitric oxide synthase, interleukin 6, BAX, and BCL. RESULTS: Recipient weight, total ischemia time, and warm ischemia time were similar between groups. Among hemodynamic and metabolic analyses, pH, central arteriovenous PCO2 difference, and AST were statistically worse in the LFS group than in the CTRL group. The same was found with endothelial nitric oxide synthase (0.41 ± 0.18 versus 1.56 ± 0.78; P = 0.02) and interleukin 6 (4.66 ± 4.61 versus 16.21 ± 8.25; P = 0.02). In the LFS group, a significant decay in the PVFr was observed in comparison with the CTRL group (0.93 ± 0.08 and 0.52 ± 0.11, respectively; P < 0.001). CONCLUSIONS: The implantation of a graft was responsible for poor hemodynamic status of the recipient 1 h after transplantation. Furthermore, the LFS group demonstrated markers of ischemia and reperfusion that were worse when compared with the CTRL group and exhibited a more significant decrease in PVF from donor to recipient.
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Authors | Daniel de Albuquerque Rangel Moreira, Ana Cristina Aoun Tannuri, Alessandro Rodrigo Belon, Maria Cecília Mendonça Coelho, Josiane Oliveira Gonçalves, Suellen Serafini, Fabiana Roberto Lima, Luciana Orsi Agostini, Raimundo Renato Guimarães, Uenis Tannuri |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 189
Issue 2
Pg. 313-20
(Jun 15 2014)
ISSN: 1095-8673 [Electronic] United States |
PMID | 24721605
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Topics |
- Animals
- Hemodynamics
- Liver
(enzymology, pathology)
- Liver Circulation
- Liver Transplantation
(adverse effects)
- Organ Size
- Pediatrics
- Random Allocation
- Reperfusion Injury
(etiology)
- Swine
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