Heat shock protein gp96 adjuvant induces T cell responses and cross-protection to a split influenza vaccine.

Abstract

The commonly used inactivated or split influenza vaccines induce only induce minimal T cell responses and are less effective in preventing heterologous virus infection. Thus, developing cross-protective influenza vaccines against the spread of a new influenza virus is an important strategy against pandemic emergence. Here we demonstrated that immunization with heat shock protein gp96 as adjuvant led to a dramatic increased antigen-specific T cell response to a pandemic H1N1 split vaccine. Notably, gp96 elicited a cross-protective CD8(+) T cell response to the internal conserved viral protein NP. Although the split pH1N1vaccine alone has low cross-protective efficiency, adding gp96 as an adjuvant effectively improved the cross-protection against challenge with a heterologous virus in mice. Our study reveals the novel property of gp96 in boosting the T cell response against conserved epitopes of influenza virus and its potential use as an adjuvant for human pre-pandemic inactivated influenza vaccines against different viral subtypes.

• Authors: Ying Ju, Hongxia Fan, Jun Liu, Jun Hu, Xinghui Li, Changfei Li, Lizhao Chen, Qiang Gao, George F Gao, Songdong Meng
• Journal: Vaccine (Vaccine) Vol. 32 Issue 23 Pg. 2703-11 (May 13 2014) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 24699472 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Antibodies, Viral
• Heat-Shock Proteins
• Influenza Vaccines

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Antibodies, Viral (blood)
• CD8-Positive T-Lymphocytes (immunology)
• Cross Protection
• Female
• Heat-Shock Proteins (pharmacology)
• Hemagglutination Inhibition Tests
• Immunity, Cellular
• Immunity, Humoral
• Influenza A Virus, H1N1 Subtype
• Influenza Vaccines (immunology)
• Mice, Inbred BALB C
• Neutralization Tests
• Orthomyxoviridae Infections (prevention & control)