Identification and analysis of differential miRNAs in PK-15 cells after foot-and-mouth disease virus infection.

Abstract

The alterations of MicroRNAs(miRNAs) in host cell after foot-and-mouth disease virus (FMDV) infection is still obscure. To increase our understanding of the pathogenesis of FMDV at the post-transcriptional regulation level, Solexa high-throu MicroRNAs (miRNAs) play an important role both in the post-transcriptional regulation of gene expression and host-virus interactions. Despite investigations of miRNA expression ghput sequencing and bioinformatic tools were used to identify differentially expressed miRNAs and analyze their functions during FMDV infection of PK-15 cells. Results indicated that 9,165,674 and 9,230,378 clean reads were obtained, with 172 known and 72 novel miRNAs differently expressed in infected and uninfected groups respectively. Some of differently expressed miRNAs were validated using stem-loop real-time quantitative RT-PCR. The GO annotation and KEGG pathway analysis for target genes revealed that differently expressed miRNAs were involved in immune response and cell death pathways.

• Authors: Ke-Shan Zhang, Yong-Jie Liu, Han-Jin Kong, Wei-Wei Cheng, You-Jun Shang, Hong Tian, Hai-Xue Zheng, Jian-Hong Guo, Xiang-Tao Liu, Xian-Tao Liu
• Journal: PloS one (PLoS One) Vol. 9 Issue 3 Pg. e90865 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24675746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MicroRNAs

Topics

• Animals
• Cell Line
• Computational Biology (methods)
• Foot-and-Mouth Disease (genetics, virology)
• Foot-and-Mouth Disease Virus (physiology)
• Gene Expression Profiling
• Gene Expression Regulation
• Gene Library
• Gene Ontology
• High-Throughput Nucleotide Sequencing
• MicroRNAs (genetics)
• Molecular Sequence Annotation
• Reproducibility of Results