Abstract | BACKGROUND & AIMS: The metabolic pathway disturbances associated with hepatocellular carcinoma (HCC) remain unsatisfactorily characterized. Determination of the metabolic alterations associated with the presence of HCC can improve our understanding of the pathophysiology of this cancer and may provide opportunities for improved disease monitoring of patients at risk for HCC development. To characterize the global metabolic alterations associated with HCC arising from hepatitis C (HCV)-associated cirrhosis using an integrated non-targeted metabolomics methodology employing both gas chromatography/mass spectrometry (GC/MS) and ultrahigh-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC/MS-MS). METHODS: The global serum metabolomes of 30 HCC patients, 27 hepatitis C cirrhosis disease controls and 30 healthy volunteers were characterized using a metabolomics approach that combined two metabolomics platforms, GC/MS and UPLC/MS-MS. Random forest, multivariate statistics and receiver operator characteristic analysis were performed to identify the most significantly altered metabolites in HCC patients vs. HCV- cirrhosis controls and which therefore exhibited a close association with the presence of HCC. RESULTS: CONCLUSIONS:
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Authors | Asem I Fitian, David R Nelson, Chen Liu, Yiling Xu, Miguel Ararat, Roniel Cabrera |
Journal | Liver international : official journal of the International Association for the Study of the Liver
(Liver Int)
Vol. 34
Issue 9
Pg. 1428-44
(Oct 2014)
ISSN: 1478-3231 [Electronic] United States |
PMID | 24661807
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Amino Acids
- Bile Acids and Salts
- Dicarboxylic Acids
- Hydroxyeicosatetraenoic Acids
- Xanthine
- 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
- 15-hydroxy-5,8,11,13-eicosatetraenoic acid
- Sphingosine
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Topics |
- 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
(blood)
- Amino Acids
(blood)
- Bile Acids and Salts
(blood)
- Carcinoma, Hepatocellular
(blood, etiology)
- Chromatography, High Pressure Liquid
(methods)
- Dicarboxylic Acids
(blood)
- Gas Chromatography-Mass Spectrometry
(methods)
- Hepatitis C
(blood, complications)
- Humans
- Hydroxyeicosatetraenoic Acids
(blood)
- Liver Cirrhosis
(blood, etiology)
- Liver Neoplasms
(blood, etiology)
- Metabolome
(physiology)
- Metabolomics
(methods)
- Multivariate Analysis
- ROC Curve
- Sphingosine
(blood)
- Tandem Mass Spectrometry
(methods)
- Xanthine
(blood)
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