Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells.

Abstract

PURPOSE:

In the present study, we investigated effects of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells in vitro.

MATERIALS AND METHODS:

Human ovarian cancer HO-8910 cell as a cancer model cell was incubated with hypocrellin B at a concentration of 2.5 μM for 5 h and irradiated by light from a light-emitting diodes (LED) source. Cell apoptosis was analyzed by flow cytometry with annexin V/propidium iodide (PI) staining and nuclear staining 6 h after hypocrellin B photoirradiation. Cell adhesion was assessed using the 3-(4, 5-dimthylthiazol-2-yl)-2, 5 diphenyl-tetrazolium bromide (MTT) assay 4 h after photodynamic treatment. Cell migration was measured 48 h after photodynamic treatment.

RESULTS:

Flow cytometry with annexin V/PI staining showed that early apoptotic and late apoptotic (necrotic) rates following photodynamic therapy with hypocrellin B markedly increased to 16.40% and 24.67%, respectively. Nuclear staining found nuclear condensation and typical apoptotic body in the treated cells. The number of cell migration was significantly decreased to 183 ± 28 after photodynamic therapy with hypocrellin B (p < 0.01). Light irradiation alone and hypocrellin B alone had no significant effect on cell migration. The cell adhesion inhibitory rate due to photodynamic action of hypocrellin B was 53.2 ± 1.8%, significantly higher than 2.7 ± 2.1% of light treatment alone and 1.0 ± 0.4% of hypocrellin B treatment alone (p < 0.01).

CONCLUSION:

The findings demonstrated that photodynamic therapy with hypocrellin B remarkably induced apoptosis and inhibited adhesion and migration of cancer cells in vitro.

• Authors: Yuan Jiang, Albert Wingnang Leung, Xinna Wang, Hongwei Zhang, Chuanshan Xu
• Journal: International journal of radiation biology (Int J Radiat Biol) Vol. 90 Issue 7 Pg. 575-9 (Jul 2014) ISSN: 1362-3095 [Electronic] England
• PMID: 24661233 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Photosensitizing Agents
• Quinones
• Perylene
• hypocrellin B

Topics

• Apoptosis (drug effects, radiation effects)
• Cell Adhesion (drug effects, radiation effects)
• Cell Line, Tumor
• Cell Movement (drug effects, radiation effects)
• Cell Nucleus (drug effects, radiation effects)
• Female
• Humans
• Ovarian Neoplasms (pathology)
• Perylene (analogs & derivatives, pharmacology)
• Photochemotherapy
• Photosensitizing Agents (pharmacology)
• Quinones (pharmacology)