Abstract | BACKGROUND: The Organ Procurement and Transplantation Network (OPTN) has formulated criteria for the selection of donors for intestinal transplantation. To date, however, no study has correlated histologic findings of intestinal injury with the OPTN criteria. We aimed to describe histopathologic and molecular features of allograft injury in relation to donor conditions defined by the OPTN criteria. MATERIALS AND METHODS: RESULTS: Ninety-seven samples (52 jejunum/45 ileum) were recovered from 59 donors (46 DBD/13 DCD). The OPTN criterion cold ischemia time correlated with histologic injury (Park score) to which the jejunum appeared more susceptible than the ileum. Claudin-3 staining was higher, and heat shock protein 70 expression lower in donors meeting the OPTN criteria compared with donors not meeting the criteria and in DBD versus DCD. In DBD donors, interleukin 6 expression was higher compared with DCD donors and inversely related to C-reactive protein. CONCLUSIONS: Our multiparameter analysis suggests that the OPTN criteria can be discriminative concerning intestinal graft quality. Our data suggest that DCD intestinal allografts are qualitatively inferior and that the jejunum is more sensitive to ischemia than the ileum.
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Authors | Anne Margot C Roskott, Wouter T van Haaften, Henri G D Leuvenink, Rutger J Ploeg, Harry van Goor, Tjasso Blokzijl, Petra J Ottens, Gerard Dijkstra, Vincent B Nieuwenhuijs |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 189
Issue 1
Pg. 143-51
(Jun 01 2014)
ISSN: 1095-8673 [Electronic] United States |
PMID | 24655665
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- CLDN3 protein, human
- Claudin-3
- Interleukin-6
- C-Reactive Protein
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Topics |
- Adolescent
- Adult
- Aged
- C-Reactive Protein
(genetics, metabolism, standards)
- Child
- Child, Preschool
- Claudin-3
(genetics, metabolism, standards)
- Endotoxemia
(etiology, pathology)
- Humans
- Ileum
(metabolism, pathology, transplantation)
- Infant
- Interleukin-6
(biosynthesis, genetics, standards)
- Jejunum
(metabolism, pathology, transplantation)
- Middle Aged
- Organ Transplantation
(adverse effects, standards)
- Tissue and Organ Procurement
(standards)
- Young Adult
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