Abstract |
Accumulating evidence has indicated that microRNAs ( miRNAs or miRs) play important roles in the developing rat brain. In this study, we investigated the role of miRNAs in the brains of immature (20-80 days) rats with liquid nitrogen lesion-induced focal cortical dysplasia. miRNA microarray demonstrated that the expression of miR-139‑5p was associated with cortical development. Bioinformatic analysis and luciferase assays revealed that the Lis1 gene is a likely target of miR-139‑5p. It is known that Lis1 plays a role in cell proliferation and migration and can lead to cortical dysplasia when mutated. Our data demonstrated an inhibitory effect of miR-139-5p on the expression of Lis1 in PC12 cells 24 h following transfection with pre-miR-139-5p. However, when the PC12 cells were transfected with anti-miR-139-5p, an increase was observed in the expression of Lis1. Cell migration assay revealed that miR-139-5p significantly inhibited the migration of PC12 and HCN-2 cells treated with or without Lis1 protein. In addition, a rat model of focal cortical dysplasia was established, wherein miR-139-5p was administered and Lis1 expression was found to be markedly reduced. Moreover, the injured cortex showed a certain degree of recovery following the administration of miR‑139-5p, demonstrating that the reduction in miR-139-5p was at least partially responsible for the upregulation of Lis1 in the rat brains. Our data suggest that miR-139-5p modulates cortical neuronal migration by targeting Lis1.
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Authors | Yanjun Huang, Jiao Jiang, Guo Zheng, Jing Chen, Haiying Lu, Hu Guo, Chunfeng Wu |
Journal | International journal of molecular medicine
(Int J Mol Med)
Vol. 33
Issue 6
Pg. 1407-14
(Jun 2014)
ISSN: 1791-244X [Electronic] Greece |
PMID | 24647639
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MicroRNAs
- Nerve Tissue Proteins
- Pafah1b1 protein, rat
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Topics |
- Animals
- Cell Movement
(genetics, physiology)
- Disease Models, Animal
- Epilepsy
- Malformations of Cortical Development, Group I
(genetics, metabolism)
- MicroRNAs
(genetics, metabolism, physiology)
- Nerve Tissue Proteins
(genetics, metabolism)
- PC12 Cells
- Rats
- Rats, Sprague-Dawley
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