Spectrum-effect relationships between UPLC fingerprints and bioactivities of crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi) and its three processed products on mitochondrial growth coupled with canonical correlation analysis.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi), together with its processed products, including Yanfuzi, Heishunpian and Paofupian, are commonly applied in clinic using for thousands of years, such as collapse, syncope, rheumatic fever, painful joints and various tumors.

AIM OF THE STUDY:

To explore the different effects of Fuzi and its processed products on energy metabolism, with mitochondria as the model with the aim of guiding the clinical use of Fuzi and its products. fingerprints of Fuzi, Yanfuzi, Heishunpian and Paofupian were established by Ultra-high Performance Liquid Chromatography (UPLC) and effects of Fuzi and its processed products on rat's liver׳s mitochondrial metabolism were studied by microcalorimetry. Spectrum-effect relationships between UPLC fingerprints and energy metabolism of mitochondria were investigated using canonical correlation analysis (CCA).

RESULTS:

Because of their inherent differences in chemical compositions, the main activities of energy metabolism of mitochondria were different among Fuzi and its processed products. The potential bioactivity sequence of the tested products was Fuzi>Heishunpian>Paofupian>Yanfuzi. RESULTS of CCA showed that compounds mesaconitine, benzoylaconitine, and benzoylhypacoitine might be the principal active components.

CONCLUSION:

Altogether, this work provides a general model of combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Fuzi and its processed products which can offer some references for detecting principal components of traditional Chinese medicine on bioactivity to mitochondrial growth.

• Authors: Quanfu Zheng, Yanling Zhao, Jiabo Wang, Tiantian Liu, Bo Zhang, Man Gong, Jianyu Li, Honghong Liu, Bin Han, Yaming Zhang, Xueai Song, Yonggang Li, Xiaohe Xiao
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 153 Issue 3 Pg. 615-23 (May 14 2014) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 24632114 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Plant Preparations
• Aconitine

Topics

• Aconitine (analogs & derivatives, pharmacology)
• Aconitum
• Animals
• Calorimetry
• Chromatography, High Pressure Liquid
• Male
• Medicine, Chinese Traditional
• Mitochondria, Liver (drug effects, metabolism)
• Plant Preparations (pharmacology)
• Plant Roots
• Rats, Sprague-Dawley